Effect of temperature on water transport through aquaporins
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The mean effective water self-diffusion coefficient in maize root segments under the effect of aquaporin blocker (mercuric chloride, 0.1 mM) was measured using the spin-echo NMR method with pulsed magnetic field gradient within the temperature range from 10 to 35 °C. HgCl2 caused the reduction in water diffusion by 30 % as compared to the control samples. Temperature dependences of water self-diffusion coefficients showed two linear regions with different values of Q10 and activation energy, Ea. As the temperature reduced from 20 to 10 °C, Ea values calculated from the Arrhenius plots were close to those of bulk water (20 ± 3 kJ mol−1) and slightly changed for the sample pretreated HgCl2. Within the temperature range from 25 to 35 °C the slope of temperature dependences became steeper and Ea values were 31 ± 3 kJ mol−1 for the control and 40 ± 4 kJ mol−1 for the treated sample. In the vicinity of 20 °C, the temperature dependence of water diffusion via the mercury-sensitive water channels showed extreme value. In the region, the specific area of the mercury-sensitive aquaporins was 0.004 % of the total cell surface area. The data indicate that water transfer via aquaporins is sensitive to temperature, and the contributions of the transmembrane pathways (aquaporins, lipid bilayer) differ in different temperature ranges.
Additional key wordsactivation energy nuclear magnetic resonance permeability transmembrane transfer Zea mays
effective diffusion coefficient of water
coefficient of diffusion water permeability of membranes
relative echo amplitude
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This research was supported by grant No. 08-04-01258 from Russian Foundation for Basic Research.
- Alleva, K., Niemietz, C.M., Sutka, M., Maurel, C., Parisi, M., Tyerman, S.D., Amodeo, G.: Plasma membrane of Beta vulgaris storage root shows high water channel activity regulated by cytoplasmic pH and a dual range of calcium concentrations. — J. exp. Bot. 57: 609–621, 2006.CrossRefPubMedGoogle Scholar
- Anisimov, A.V., Ionenko, I.F., Romanov, A.V.: Spin-echo NMR study of the translational water diffusion selectively along the apoplast and the cytoplasmic and vacuolar symplasts of plants. — Biophysics 49: 816–821, 2004.Google Scholar
- Finkelstein, A.: Water Movement through Lipid Bilayers, Pores and Plasma Membranes. Theory and Reality. Vol. 4. — Wiley-Interscience Publishers, New York 1987.Google Scholar
- Schütz, K., Tyerman, S.D.: Water channels in Chara coralline. — J. exp. Bot. 48: 1511–1518, 1997.Google Scholar
- Van Dusschoten, D., De Jager, P.A., Van As, H.: Extracting diffusion constants from echo-time-dependent PFG NMR data using relaxation-time information. — J. Magnetic. Resonance 116: 22–28, 1995.Google Scholar