Biologia Plantarum

, 53:793 | Cite as

Development of novel chloroplast microsatellite markers for Cucumis from sequence database

Brief Communication
First Online:
Received:
Accepted:

Abstract

The development of chloroplast microsatellite (cpSSR) markers in Cucumis species and analysis of their polymorphism and transferability were reported. Fifteen microsatellite markers, represented by mononucleotide repeats, were developed from the complete sequence of Cucumis sativus chloroplast genome. Intraspecific variation was successfully detected in C. sativus and C. melo and revealed mean 1.6 and 1.9 alleles per cpSSR locus, respectively. With the exception of two exon region-located cpSSR markers being monomorphic, each of the others amplified polymorphic fragments in C. sativus or C. melo. A total of 34 polymorphic loci were detected with these cpSSR markers in the two species. Transferability of the newly developed cpSSR markers was checked on an additional set of 41 Cucurbitaceae accessions (belonging to 12 different species), and except for two markers with no amplification in Cucurbita maxima, the others could be transferable to all the accessions tested. Of the 15 cpSSR markers, 14 markers generated fragments with expected band sizes and 13 markers detected interspecific polymorphism among the accessions. Intraspecific polymorphism was also observed within four Cucurbitaceae species excluding C. sativus and C. melo.

Additional key words

Cucurbitaceae monomorphism polymorphism transferability 

Abbreviations

SSR

microsatellite or simple sequence repeat

cpSSR

chloroplast microsatellite

RFLP

restriction fragment length polymorphism

CTAB

cetyltrimethylammonium bromide

This is a preview of subscription content, log in to check access.

References

  1. Birky, C.W.: Evolution and population genetics of organelle genes: mechanisms and models. — In: Gottlieb, L.D., Jain, S.K. (ed.): Plant Evolutionary Biology. Pp. 23–53. Chapman & Hall, London 1988.Google Scholar
  2. Bryan, G.J., McNicoll, J., Ramsay, G., Meyer, R.C., De Jong, W.S.: Polymorphic simple sequence repeat markers in chloroplast genomes of Solanaceous plants. — Theor. appl. Genet. 99: 859–867, 1999.CrossRefGoogle Scholar
  3. Danin-Poleg, Y., Reis, N., Tzuri, G., Katzir, N.: Development and characterization of microsatellite markers in Cucumis. — Theor. appl. Genet. 102: 61–72, 2001.CrossRefGoogle Scholar
  4. Ishii, T., McCouch, S.R.: Microsatellites and microsynteny in the chloroplast genomes of Oryza and eight other Gramineae species. — Theor. appl. Genet. 100: 1257–1266, 2000.CrossRefGoogle Scholar
  5. Kong, Q., Xiang, C., Yu, Z.: Development of EST-SSRs in Cucumis sativus from sequence database. — Mol. Ecol. Notes 6: 1234–1236, 2006.CrossRefGoogle Scholar
  6. Kong, Q., Xiang, C., Yu, Z., Zhang, C., Liu, F., Peng, C., Peng, X.: Mining and charactering microsatellites in Cucumis melo expressed sequence tags from sequence database. — Mol. Ecol. Notes 7: 281–283, 2007.CrossRefGoogle Scholar
  7. McGrath, S., Hodkinson, T.R., Salamin, N., Barth, S.: Development and testing of novel chloroplast microsatellite markers for Lolium perenne and other grasses (Poaceae) from de novo sequencing and in silico sequences. — Mol. Ecol. Notes 6: 449–452, 2006.CrossRefGoogle Scholar
  8. Murray, M.G., Thompson, W.F.: Rapid isolation of high molecular weight plant DNA. — Nucl. Acids Res. 10: 4321–4325, 1980.CrossRefGoogle Scholar
  9. Nei, M. (ed.): Molecular Evolutionary Genetics. — Columbia University Press, New York 1987.Google Scholar
  10. Provan, J., Corbett, G., McNicol, J.W, Powell, W.: Chloroplast DNA variability in wild and cultivated rice (Oryza spp.) revealed by polymorphic chloroplast simple sequence repeats. — Genome 40: 104–410, 1997.CrossRefPubMedGoogle Scholar
  11. Provan, J., Powell, W., Hollingsworth, P.M.: Chloroplast microsatellites: new tools for studies in plant ecology and evolution. — Trends Ecol. Evol. 16: 142–147, 2001.CrossRefPubMedGoogle Scholar
  12. Provan, J., Russell, J.R., Booth, A., Powell, W.: Polymorphic chloroplast simple sequence repeat primers for systematic and populations studies in the genus Hordeum. — Mol. Ecol. 8: 505–511, 1999.CrossRefPubMedGoogle Scholar
  13. Skálová, D., Dziechciarková, M., Lebeda, A., Křístková, E., Navrátilová, B.: Interspecific hybridization of Cucumis anguria and C. zeyheri via embryo-rescue. — Biol. Plant. 52: 775–778, 2008.CrossRefGoogle Scholar
  14. Watcharawongpaiboon, N., Chunwongse, J.: Development and characterization of microsatellite markers from an enriched genomic library of cucumber (Cucumis sativus). — Plant Breed. 127: 74–81, 2008.Google Scholar
  15. Weising, K., Gardner, R.C.: A set of conserved PCR primers for the analysis of simple sequence repeat polymorphisms in chloroplast genomes of dicotyledonous angiosperms. — Genome 42: 9–19, 1999.CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  1. 1.College of HorticultureHenan Agricultural UniversityZhengzhouP.R. China
  2. 2.College of HorticultureNortheast Agricultural UniversityHarbinP.R. China

Personalised recommendations