Biologia Plantarum

, 53:737 | Cite as

Responses of Camellia sinensis cultivars to Cu and Al stress

  • S. K. YadavEmail author
  • P. Mohanpuria
Brief Communication


The response of Camellia sinensis (L.) O. Kuntze cultivars Chinary and Assamica to Cu and Al stresses was investigated. Exposure to 100 µM CuSO4 or 100 µM AlCl3 led to accumulation of reactive oxygen species (ROS) more in Assamica than in Chinary. Proline content was higher in Chinary compared to Assamica, while chlorophyll and protein contents decreased upon Cu and Al exposure in both the cultivars. Expression of glutathione biosynthetic enzymes γ-glutamylcysteinyl synthetase (γ-ECS) and glutathione synthetase (GSHS) was elevated. Phytochelatin synthase (PCS), an enzyme involved in phytochelatins synthesis by using glutathione as a substrate was up-regulated at its transcript level more in Chinary than in Assamica. These results suggest that Chinary could be more tolerant than Assamica.

Additional key words

gene expression glutathione metabolic enzymes oxidative stress proline tea 



γ-glutamylcysteinyl synthetase




glutathione synthetase




phytochelatin synthase


reactive oxygen species


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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  1. 1.Biotechnology DivisionInstitute of Himalayan Bioresource Technology (CSIR)PalampurIndia

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