In vitro direct organogenesis and regeneration of Medicago sativa
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A rapid and efficient plant regeneration protocol for a wide range of alfalfa genotypes was developed via direct organogenesis. Through a successive excision of the newly developed apical and axillary shoots, a lot of adventitious buds were directly induced from the cotyledonary nodes when hypocotyl of explants were vertically inserted into modified Murashige and Skoog (MS) medium supplemented with 0.025 mg dm−3 thidiazuron (TDZ) and 3 mg dm−3 AgNO3. When the lower part of shoots excised from explants were immersed into the liquid medium with 1.0 mg dm−3 α-naphthaleneacetic acid (NAA) for 2 min, and then transferred to hormone free half-strength MS medium, over 83.3 % of the shoots developed roots, and all plantlets could acclimatize and establish in soil. The protocol has been successfully applied to eight genotypes, with regeneration frequencies ranging from 63.8 to 82.5 %.
Additional key wordsalfalfa cotyledonary node mature embryo silver nitrate thidiazuron vitrification
Murashige and Skoog
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- Barbulova, A., Iantcheva, A., Zhiponova, M., Vlahova, M., Atanassov, A.: Establishment of embryogenic potential of economically important Bulgarian alfalfa cultivars (Medicago sativa L.).-Biotechnol. biotechnol. Equipment 16: 55–63, 2002.Google Scholar
- Derek, W.R., White, C.V.: Prolific direct plant regeneration from cotyledons of white clover.-Plant Cell Rep. 13: 303–308, 1994.Google Scholar
- Li, S., Li, W., Yang, D.L., Cao, Z.Y.: [Advance of research vitrification in plant test-tube plantlets.]-J. Gansu agr. Univ. 38: 1–16, 2003. [In Chin.]Google Scholar
- Liang, H.M., Huang, J., Xia, Y., W, T.M., Sun, Z.X., Li, X.Y.: [Establishment of high frequency regeneration system for tissue culture of alfalfa.]-J. agr. Biotechnol. 11: 321–322, 2003. [In Chin.]Google Scholar
- Zhou, Y., Zhang, Z.Q., Zhang, J.J., Yin, L.Q.: [Study on overcome the vitrification of lettuce in genetic transformation.]-J. Jilin agr. Univ. 22: 62–64, 2000. [In Chin.]Google Scholar