Biologia Plantarum

, Volume 53, Issue 2, pp 285–293 | Cite as

Proteomic analysis of bacterial blight defence signalling pathway using transgenic rice overexpressing thaumatin-like protein

Original Papers

Abstract

Rice overexpressed thaumatin-like protein gene and the proteins from the leaf blades of 2-week-old transgenic rice seedlings were fractionated into cytosolic and membrane fractions, and separated by two-dimensional polyacrylamide gel electrophoresis and stained with Commassie brilliant blue. Among of 440 detected proteins, 5 proteins were up-regulated and 5 proteins were down-regulated by the overexpression of thaumatin-like protein. In the sense thaumatin-like protein transgenic rice and/or in rice inoculated with Xanthomonas oryzae pv. oryzae (Xo7435), 2-cys peroxiredoxin, thaumatin-like protein and glycine cleavage H protein were up-regulated, while oxygen evolving complex protein 2 was down-regulated. These results suggest that thaumatin-like protein-mediated disease resistance of rice against bacterial blight disease is the results of changes in proteins related to oxidative stress and energy metabolism in addition to changes in proteins related to defence.

Additional key words

Oryza sativa oxygen evolving complex Xanthomonas oryzae pv. oryzae 

Abbreviations

2,4-D

2,4-dichlorophenoxyacetic acid

2D-PAGE

two-dimensional polyacrylamide gel electrophoresis

MS

mass spectrometry

NAA

naphthaleneacetic acid

PR

pathogenesis-related

PVDF

polyvinylidine difluoride

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  1. 1.National Institute of Crop ScienceTsukubaJapan

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