Skip to main content

Advertisement

SpringerLink
Log in

Proteomic analysis of bacterial blight defence signalling pathway using transgenic rice overexpressing thaumatin-like protein

  • Original Papers
  • Published:
Biologia Plantarum

Abstract

Rice overexpressed thaumatin-like protein gene and the proteins from the leaf blades of 2-week-old transgenic rice seedlings were fractionated into cytosolic and membrane fractions, and separated by two-dimensional polyacrylamide gel electrophoresis and stained with Commassie brilliant blue. Among of 440 detected proteins, 5 proteins were up-regulated and 5 proteins were down-regulated by the overexpression of thaumatin-like protein. In the sense thaumatin-like protein transgenic rice and/or in rice inoculated with Xanthomonas oryzae pv. oryzae (Xo7435), 2-cys peroxiredoxin, thaumatin-like protein and glycine cleavage H protein were up-regulated, while oxygen evolving complex protein 2 was down-regulated. These results suggest that thaumatin-like protein-mediated disease resistance of rice against bacterial blight disease is the results of changes in proteins related to oxidative stress and energy metabolism in addition to changes in proteins related to defence.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

2,4-D:

2,4-dichlorophenoxyacetic acid

2D-PAGE:

two-dimensional polyacrylamide gel electrophoresis

MS:

mass spectrometry

NAA:

naphthaleneacetic acid

PR:

pathogenesis-related

PVDF:

polyvinylidine difluoride

References

  • Abbasi, F.M., Komatsu, S.: A proteomic approach to analyze salt-responsive proteins in rice leaf sheath.-Proteomics 4: 2072–2081, 2004.

    Article  PubMed  CAS  Google Scholar 

  • Ali, G.M., Komatsu, S.: Proteomic analysis of rice leaf sheath during drought stress.-J. Proteome Res. 5: 396–403, 2006.

    Article  PubMed  CAS  Google Scholar 

  • Anand, A., Zhou, T., Harold, N.T., Bikram, S.G., Willium, W.B., Subbaratnam, M.: Greenhouse and field testing of transgenic wheat plants stably expressing genes for thaumatin-like protein, chitinase and glucanase against Fusarium graminearum.-J. exp. Bot. 54: 1101–1111, 2003.

    Article  PubMed  CAS  Google Scholar 

  • Antoniw, J.F., Pierpoint, W.S.: Purification of tobacco leaf protein associated with resistance of virus infection.-Biochem. Soc. Trans. 6: 248–250, 1978.

    PubMed  CAS  Google Scholar 

  • Broglie, K., Chet, I., Holliday, M., Cressman, R., Biddle, Ph., Knowlton, S., Mauvais, C.J., Broglie, R.: Transgenic plants with enhanced resistance to the fungal pathogen Rhizoctonia solani.-Science 254: 45–52, 1991.

    Google Scholar 

  • Colditz, F., Niehaus, K., Kranjinski, F.: Silencing of PR-10-like proteins in Medicago truncatula results in an antagonistic induction of other PR proteins and in an increased tolerance upon infection with the oomycete Aphanomyces euteiches.-Planta 226: 57–71, 2007.

    Article  PubMed  CAS  Google Scholar 

  • Datta, K., Velazhahan, R., Oliva, N. Ona, I., Mew, T., Khush, G.S., Muthukrishnan, S., Datta, S.K.: Overexpression of the cloned rice thaumatin-like protein (PR5) gene in transgenic rice plants enhances environmental friendly resistance to Rhizoctonia solani causing sheath blight disease.-Theor. appl. Genet. 98: 1138–1145, 1999.

    Article  CAS  Google Scholar 

  • Dietz, K.J., Horling, F., Konig, J., Baier, M.J.: Inhibition of ascorbate peroxidase under oxidative stress in tobacco having bacterial catalase in chloroplasts.-J. exp. Bot. 53: 1321–1329, 2002.

    Article  PubMed  CAS  Google Scholar 

  • Eubel, H., Jansch, L., Braun, H.P.: New insights into the respiratory chain of plant mitochondria. Supercomplexes and a unique composition of complex II.-Plant Physiol. 133: 274–286, 2003.

    Article  PubMed  CAS  Google Scholar 

  • Ezuka, A., Kaku, H.: A historical review of bacterial blight of rice.-Bull. nat. Inst. Agrobiol. Resour. 15: 1–207, 2000.

    CAS  Google Scholar 

  • Harding, S.A., Oh, S.H., Roberts, D.M.: Transgenic tobacco expressing a foreign calmodulin gene shows an enhanced production of active oxygen species.-EMBO J. 16: 1137–1144, 1997.

    Article  PubMed  CAS  Google Scholar 

  • Hiei, Y., Ohta, S., Komari, T., Kumashiro, T.: Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA.-Plant J. 6: 271–282, 1994.

    Article  PubMed  CAS  Google Scholar 

  • Hood, H.H., Helmer, G.L., Fraley, R.T., Chilton, M.D.: The hyper virulence of Agrobacterium tumefaciens A281 is encoded in a region of pTiBo542 outside of T-DNA.-J. Bacteriol. 168: 1291–1301, 1986.

    PubMed  CAS  Google Scholar 

  • Imin, N., Kerim, T., Jeremy, J.W., Barry, G.R.: Low temperature treatment at the young microspore stage induces protein changes in rice anthers.-Mol. Cell. Prot. 5: 274–292, 2006.

    Article  CAS  Google Scholar 

  • Jilao, S., Thornsburry, J.M., Elthon, T.E., Newton, K.J.: Biochemical and molecular characterization of photosystem 1 deficiency in the NCS6 mitochondrial mutant of maize.-Plant mol. Biol. 57: 303–313, 2005.

    Article  Google Scholar 

  • Kim, S.D., Kim, S.G., Hwang du, H., Kang, S.Y., Koo, S.C., Cho, M.J., Kang, K.Y.: Expression of a salt-induced protein (SALT) in suspension-cultured cells and leaves of rice following exposure to fungal elicitor and phytohormones.-Plant Cell Rep. 23: 256–262, 2004.

    Article  PubMed  CAS  Google Scholar 

  • Komatsu, S., Hirano, H.: Protein kinase activity and protein phosphorylation in rice (Oryza sativa L.) leaf.-Plant Sci. 94: 127–137, 1993.

    Article  CAS  Google Scholar 

  • Komatsu, S., Yang, G., Hayashi, N., Kaku, H., Umemura, K., Iwasaki, Y.: Alteration by a defect in rice G protein a subunit in probenazole and pathogen-induced responses.-Plant Cell Environ. 27: 947–957, 2004.

    Article  CAS  Google Scholar 

  • Konishi, H., Ishiguro, K., Komatsu, S.: A proteomics approach towards understanding blast fungus infection of rice grown under different levels of nitrogen fertilization.-Proteomics 1: 1162–1171, 2001.

    Article  PubMed  CAS  Google Scholar 

  • Laemmli, U.K.: Cleavage of structural proteins during the assembly of the head bacteriophage T4.-Nature 227: 680–685, 1974.

    Article  Google Scholar 

  • Mahmood, T., Jan, A., Kakishima, M., Komatsu, S.: Proteomic analysis of bacterial blight defense-responsive proteins in rice leaf blades.-Proteomics 6: 6053–6065, 2006.

    Article  PubMed  CAS  Google Scholar 

  • Maniatis, T., Fritsch, E.F., Sambrook, J.: Molecular Cloning: a Laboratory Manual.-Cold Spring Harbor Laboratory Press, Cold Spring Harbor 1982.

    Google Scholar 

  • Mauch, F., Reimmann, C., Freydl, E., Schaffrath, U., Dudler, R.: Characterization of rice pathogen-related protein Rir1a and regulation of the corresponding gene.-Plant mol. Biol. 38: 577–586, 1998.

    Article  PubMed  CAS  Google Scholar 

  • Murashige, T., Skoog, F.: A revise medium for rapid growth and bioassays with tobacco tissue cultures.-Physiol. Plant. 15: 473–497, 1962.

    Article  CAS  Google Scholar 

  • Nelson, D.E., Raghothama, K.G., Singh, N.K., Hasegawa, P.M., Bressan, R.A.: Analysis of structure and transcriptional activation of osmotin gene.-Plant mol. Biol. 19: 577–588, 1992.

    Article  PubMed  CAS  Google Scholar 

  • O’Farrell, H.P.: High resolution two-dimensional electrophoresis of proteins.-J. biol. Chem. 250: 4007–4021, 1975.

    PubMed  Google Scholar 

  • Rajam, M.V., Chandola, N., Saiprasad Goud, P., Singh, D., Kashyap, V., Choudhary, M.L., Sihachakr, D.: Thaumatin gene confers resistance to fungal pathogens as well as tolerance to abiotic stresses in transgenic tobacco plants.-Biol. Plant. 51: 135–141, 2007.

    Article  CAS  Google Scholar 

  • Shen, Y., Ronald, P.: Molecular determinants of disease and resistance in interactions of Xanthomonas oryzae pv. oryzae and rice.-Microb. Infect. 4: 1361–1367, 2002.

    Article  CAS  Google Scholar 

  • Stintzi, A., Heitz, T., Prasad, V., Wiedemann-Merdinoglu, S., Kauffmann, S., Geoffroy, P., Legrand, M., Fritig, B.: Plant “pathogenesis-related” proteins and their role in defense against pathogens.-Biochemie 75: 687–706, 1993.

    Article  CAS  Google Scholar 

  • Takahashi, H., Hotta, Y., Hayashi, M., Kawai-Yamada, M., Komatsu, S., Uchimiya, H.: High throughput metabolome and proteome analysis of transgenic rice plants (Oryza sativa L.).-Plant Biotechnol. 22: 47–50, 2005.

    CAS  Google Scholar 

  • Takemoto, D., Furuse, K., Doke, N., Kawakita, K.: Identification of chitinase and osmotin-like protein as actin-binding proteins in suspension-cultured potato cells.-Plant Cell Physiol. 38: 441–448, 1997.

    PubMed  CAS  Google Scholar 

  • Toki, S.: Rapid and efficient Agrobacterium mediated transformation in rice.-Plant mol. Biol. Rep. 15: 16–21, 1997.

    Article  CAS  Google Scholar 

  • Tsunezuka, H., Fujiwara, M., Kawasaki, T., Shimamoto, K.: Proteomic analysis of programmed cell death and defense signaling using the rice lesion mimic mutant cdr2.-Mol. Plant-Microbe Interact. 18: 52–59, 2005.

    Article  PubMed  CAS  Google Scholar 

  • Van Kan, J.A.L. Van De Rhee, M.D., Zuidema, D., Cornencolissen, B.J.C., Bol, J.F.: Structure of tobacco genes encoding thaumatin-like proteins.-Plant mol. Biol. 12: 153–155, 1989.

    Article  Google Scholar 

  • Van Loon, L.C., Pierpoint, W.S., Boller, T., Conejero, V.: Recommendations for naming plant pathogenesis-related proteins.-Plant mol. Rep. 12: 245–264, 1994.

    Article  Google Scholar 

  • Vigers, A.J. Wiedemann, S., Roberts, W.K., Legrand, M., Selitreeikoff, C.P., Fiting, B.: Thaumatin-like pathogenesis-related proteins are antifungal.-Plant Sci. 83: 155–161, 1992.

    Article  CAS  Google Scholar 

  • Yoshimura, S., Yoshino, R., Morihashi, T.: Lysotype of Xanthomonas oryzae and their pathogenicity.-Proc. Assoc. Plant Prot. Hokuriku (Japan) 8: 21–24, 1960.

    Google Scholar 

  • Zhu, B., Chen, T.H., Li, P.H.: Analysis of late-blight disease resistance and freezing tolerance in transgenic potato plants expressing sense and antisense genes for an osmotin-like protein.-Planta 198: 70–77, 1996.

    Article  PubMed  CAS  Google Scholar 

  • Zou, J., Rodriguez-Zas, S., Aldea, M., Li, M., Zhu, J., Gonzalez, D.O., Vodkin, L.O., DeLucia, E., Clough, S.J.: Expression profiling soybean response to Pseudomonas syringae reveals new defense-related genes and rapid HR-specific downregulation of photosynthesis.-Mol. Plant-Microbe Interact. 18: 1161–1174, 2005.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. National Institute of Crop Science, Tsukuba, 3058518, Japan

    T. Mahmood, A. Jan & S. Komatsu

Authors
  1. T. Mahmood
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. Jan
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. S. Komatsu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to S. Komatsu.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Mahmood, T., Jan, A. & Komatsu, S. Proteomic analysis of bacterial blight defence signalling pathway using transgenic rice overexpressing thaumatin-like protein. Biol Plant 53, 285–293 (2009). https://doi.org/10.1007/s10535-009-0052-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10535-009-0052-9

Additional key words

Search

Navigation