Biologia Plantarum

, Volume 53, Issue 2, pp 243–248 | Cite as

Salt stress effects on growth, pigments, proteins and lipid peroxidation in Salicornia persica and S. europaea

  • M. Aghaleh
  • V. NiknamEmail author
  • H. Ebrahimzadeh
  • K. Razavi
Original Papers


The effects of NaCl stress on growth, water status, contents of protein, proline, malondialdehyde (MDA), various sugars and photosynthetic pigments were investigated in seedlings of Salicornia persica and S. europaea grown in vitro. Seeds were germinated under NaCl (0, 100, 200, 300, 400, 500 and 600 mM) on Murashige and Skoog medium for 45 d. The shoot growth of both species increased under low NaCl concentration (100 mM) and then decreased with increasing NaCl concentrations. In contrast to S. persica, root length in S. europaea reduced steadily with an increase in salinity. Proline content in S. persica was higher than in S. europaea at most NaCl concentrations. Proline, reducing saccharide, oligosaccharide and soluble saccharide contents increased under salinity in both species. In contrast, contents of proteins and polysaccharides reduced in both species under salt stress. MDA content remained close to control at moderate NaCl concentrations (100 and 200 mM) and increased at higher salinities. MDA content in S. europaea was significantly higher than S. persica at higher salinities. Salt treatments decreased K+ and P contents in seedlings of both species. Significant reduction in contents of chlorophylls and carotenoids due to NaCl stress was also observed in seedlings of both species. Some differences appeared between S. persica and S. europaea concerning proteins profile. On the basis of the data obtained, S. persica is more salt-tolerant than S. europaea.

Additional key words

halophyte in vitro culture free proline MDA photosynthetic pigment RWC salinity saccharides 








MS medium

Murashige and Skoog medium


relative water content


water content


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  1. Agarwal, S., Pandey, V.: Antioxidant enzyme responses to NaCl stress in Cassia angustifolia.-Biol. Plant. 48: 555–560, 2004.CrossRefGoogle Scholar
  2. Akhani, H.: Salicornia persica Akhani (Chenopodiaceae), a remarkable new species from central Iran.-Linzer Biol. Bietr. 35: 607–612, 2003.Google Scholar
  3. Ajmal Khan, M., Ungar, I.A., Showalter, A. M.: The effect of salinity on growth, water status, and ion content of leaf succulent perennial halophyte, Suaeda fruticosa (L.) Forssk.-J. arid Environ. 45: 73–84, 2000.CrossRefGoogle Scholar
  4. Amor, N.B., Hamed, K.B., Debez, A.: Physiological and antioxidant response of the perennial halophyte Crithmum maritimum to salinity.-Plant Sci. 168: 889–899, 2005.CrossRefGoogle Scholar
  5. Ayala, F., O’Leary, J.W.: Growth and physiology of Salicornia bigelovii Torr. at suboptimal salinity.-Int. J. Plant Sci. 156: 197–202, 1995.CrossRefGoogle Scholar
  6. Bates, L.S., Walderd, R.P., Teare, I.D.: Rapid determination of free proline for water stress studies.-Plant Soil 39: 205–208, 1973.CrossRefGoogle Scholar
  7. Binzel, M.L., Hess, F.D., Bressan, R.A., Hasegawa, P. M.: Mechanism of adaptation to salinity in cultured glycophyte cells.-In: Cherry, J.H. (ed.): Biochemical and Physiological Mechanisms Associated with Environmental Stress Tolerance. Pp. 139–157. Springer-Verlag, Berlin 1989.Google Scholar
  8. Bohnert, H.J., Nelson, D.E., Jensen, R.J.: Adaptation to environmental stress.-Plant Cell 7: 1099–1011, 1995.PubMedCrossRefGoogle Scholar
  9. Bradford, M.M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principles of protein-dye binding.-Anal. Biochem. 72: 248–254, 1976.PubMedCrossRefGoogle Scholar
  10. Bradley, P.H., Morris, J.T.: Relative importance of ion exclusion, secretion and accumulation in Spartina alterniflora Loisel.-J. exp. Bot. 42: 1525–1532, 1991.CrossRefGoogle Scholar
  11. Dubey, R.S., Singh, A.K.: Salinity induces accumulation of soluble sugars and alters the activity of sugar metabolizing enzymes in rice plants.-Biol. Plant. 42: 233–239, 1999.CrossRefGoogle Scholar
  12. Dubois, M., Gille, K.A., Hamilton, J.K., Rebers, P.A., Smith, F.: Colorimetric method for determination of sugars and related substance.-Anal. Chem. 28: 350–356, 1956.CrossRefGoogle Scholar
  13. Elshintinawy, F., Elshourbagy, M.N.: Alleviation of changes in protein metabolism in NaCl-stressed wheat seedlings by thiamine.-Biol. Plant. 44: 541–545, 2001.CrossRefGoogle Scholar
  14. Hassanein, A.M.: Alterations in protein and esterase patterns of peanut in response to salinity stress.-Biol. Plant. 42: 241–248, 1999.CrossRefGoogle Scholar
  15. Heidari-Sharifabad, H., Mirzaie-Nadoushan, H.: Salinity-induced growth and some metabolic changes in three Salsola species.-J. arid Environ. 67: 715–720, 2006.CrossRefGoogle Scholar
  16. Heath, R.L., Packer, L.: Photoperoxidation in isolated chloroplasts.-Arch. Biochem. Biophys. 125: 189–198, 1968.PubMedCrossRefGoogle Scholar
  17. Hernandez, J.A., Almansa, M.S.: Short-term effects of salt stress on antioxidant systems and leaf water relations of pea leaves.-Physiol. Plant 115: 251–257, 2002.PubMedCrossRefGoogle Scholar
  18. Kato, M., Shimizu, S.: Chlorophyll metabolism in higher plants. VI. Involvement of peroxidase in chlorophyll degeneration.-Plant Cell Physiol. 26: 1291–1301, 1985.Google Scholar
  19. Laemmli, U. K.: Cleavage of structural proteins during the assembly of the head bacteriophage T4.-Nature 227: 680–685, 1970.PubMedCrossRefGoogle Scholar
  20. Lichtenthaler, H., Wellburm, A.R.: Determination of total carotenoids and chlorophyll a and b of leaf extracts in different solvents.-Biochem. Soc. Trans. 603: 591–593, 1983.Google Scholar
  21. McCoy, T.J.: Tissue culture evaluation of NaCl tolerance in Medicago sativa species: cellular versus whole plant response.-Plant Cell Rep. 6: 31–34, 1987.CrossRefGoogle Scholar
  22. Meloni, D.A., Oliva, M.A., Martinez, C.A., Cambraia, J.: Photosynthesis and activity of superoxide dismutase, peroxidase, and gluthatione reductase in cotton under salt stress.-Environ. exp. Bot. 49: 69–76, 2003.CrossRefGoogle Scholar
  23. Moghaieb, R.E.A., Saneoka, H., Fujita, K.: Effect of salinity on osmotic adjustment, glycinebetaine accumulation and the betaine aldehyde dehydrogenase gene expression in two halophyte plants, Salicornia europaea and Suaeda maritima.-Plant Sci. 166: 1345–1349, 2004.CrossRefGoogle Scholar
  24. Munoz, G.E., Marin, K., Gonzalez, C.: Polypeptide profile in Prosopsis seedlings growing in saline conditions.-Phyton 61: 17–24, 1997.Google Scholar
  25. Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassay with tobacco tissue cultures.-Plant Physiol. 15: 473–497, 1962.CrossRefGoogle Scholar
  26. Nelson, N.: A Photometric adaptation of the Somogyi method for the determination of glucose.-J. biol. Chem. 153: 374–380, 1944.Google Scholar
  27. Niknam, V., Bagherzadeh, M., Ebrahimzadeh, H., Sokhansanj, A.: Effect of NaCl on biomass and content of sugars, proline and proteins in seedlings and leaf explants of Nicotiana tabacum grown in vitro.-Biol. Plant. 48: 613–615, 2004.CrossRefGoogle Scholar
  28. Niknam, V., Razavi, N., Ebrahimzadeh, H., Sharifizadeh, B.: Effect of NaCl on biomass, protein and proline contents, and antioxidant enzymes in seedlings and calli of two Trigonella species.-Biol. Plant. 50: 591–596, 2006.CrossRefGoogle Scholar
  29. O’Leary, J.W., Glenn, E.P., Watson, M.C.: Agriculture production of halophytes irrigated with seawater.-Plant Soil 89: 311–321, 1985.CrossRefGoogle Scholar
  30. Parida, A.K., Das, A.B.: Effects of NaCl stress on nitrogen and phosphorous metabolism in a true mangrove Bruguiera parviflora grown under hydroponic culture.-J. Plant Physiol. 161: 921–928, 2004.PubMedCrossRefGoogle Scholar
  31. Parida, A.K., Das, A.B., Das, P.: NaCl stress causes changes in photosynthetic pigments, proteins and other metabolic components in the leaves of a true mangrove, Bruguiera parviflora, in hydroponic cultures.-J. Plant Biol. 45: 28–36, 2002.CrossRefGoogle Scholar
  32. Sairam, R.K., Srivastava, G.C., Agarwal, S., Meena, R.C.: Differences in antioxidant activity in response to salinity stress in tolerant and susceptible wheat genotypes.-Biol. Plant. 49: 85–91, 2005.CrossRefGoogle Scholar
  33. Sanchez, F.J., Manzanares, M., Andres, E. F., Tenorio, J.L., Ayerbe, L., Andres, E.F.: Turgor maintenance, osmotic adjustment and soluble sugar and proline accumulation in 49 pea cultivars in response to water stress.-Field Crops Res. 59: 225–235, 1998.CrossRefGoogle Scholar
  34. Scandalios, J.G.: Oxygen stress and superoxide dismutase.-Plant Physiol. 101: 7–12, 1993.PubMedGoogle Scholar
  35. Shalata, A., Mitova, V., Volokita, M., Guy, M., Tal, M.: Response of the cultivated tomato and its wild salt-tolerant relative Lycopersicon pennelli to salt-dependent oxidative stress.-Physiol. Plant. 112: 487–494, 2001.PubMedCrossRefGoogle Scholar
  36. Wheatherley, P.E.: Studies in the water relations of cotton plants. I. The field measurement of water deficit in leaves.-New Phytol. 49: 81–87, 1950.CrossRefGoogle Scholar
  37. Winicov, I., Bastola, D.R.: Salt tolerance in crop plants: new approaches through tissue culture and gene regulation.-Acta Physiol. Plant. 19: 435–449, 1997.CrossRefGoogle Scholar
  38. Zhao, K.F., Feng, L.T.: Halophyte Resources in China.-Science Press, Beijing 2001.Google Scholar

Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • M. Aghaleh
    • 1
  • V. Niknam
    • 1
    Email author
  • H. Ebrahimzadeh
    • 1
  • K. Razavi
    • 2
  1. 1.School of BiologyUniversity College of Science, University of TehranTehranI.R. Iran
  2. 2.Department of Plant BiotechnologyNational Institute for Genetic Engineering and BiotechnologyTehranI.R. Iran

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