Biologia Plantarum

, Volume 53, Issue 1, pp 164–166

Development and evaluation of microsatellite markers in Phoenix dactylifera L. and their transferability to other Phoenix species

  • A. Akkak
  • V. Scariot
  • D. Torello Marinoni
  • P. Boccacci
  • C. Beltramo
  • R. Botta
Brief Communication
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Abstract

Forty one simple sequence repeats were isolated from two microsatellite enriched libraries of date palm (Phoenix dactylifera L.). After screening, 17 selected microsatellite loci were characterized and evaluated on a set of 31 cultivars and clones from Algerian and Californian germplasm. All primer pairs produced an amplification product of the expected size and detected high polymorphism among the analysed samples. These nuclear simple sequence repeat (SSR) markers are expected to be a very effective tool for evaluating genetic diversity in date palm germplasm. Acrosstaxa amplification showed the usefulness of most SSR markers in 14 other species across the genus Phoenix.

Additional key words

across-taxa transferability date palm simple sequence repeats 

Abbreviation

SSRs

simple sequence repeats

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • A. Akkak
    • 1
  • V. Scariot
    • 2
  • D. Torello Marinoni
    • 3
  • P. Boccacci
    • 3
  • C. Beltramo
    • 3
  • R. Botta
    • 3
  1. 1.Dipartimento Scienze Agro-Ambientali, Chimica e Difesa VegetaleUniversità degli Studi di FoggiaFoggiaItaly
  2. 2.Dipartimento di Agronomia, Selvicoltura e Gestione del TerritorioUniversità di TorinoGrugliascoItaly
  3. 3.Dipartimento di Colture ArboreeUniversità di TorinoGrugliascoItaly

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