Biologia Plantarum

, Volume 52, Issue 4, pp 767–770 | Cite as

Effects of 28-homobrassinolide on nickel uptake, protein content and antioxidative defence system in Brassica juncea

  • P. Sharma
  • R. Bhardwaj
  • N. Arora
  • H. K. Arora
  • A. Kumar
Brief Communication


The effects of 28-homobrassinolide (HBL) on nickel uptake, protein content and activities of antioxidative enzymes were determined in the seedlings of Brassica juncea L. The seeds were treated with different concentrations (0, 0.01, 1 and 100 nM) of HBL for 8 h and then sown in the Petri plates containing various concentrations (0, 25, 50 and 100 mg dm−3) of nickel. After 7 d, observations were made on shoot and root length, Ni uptake, protein content and activities of antioxidative enzymes (guaiacol peroxidase, catalase, glutathione reductase, ascorbate peroxidase and superoxide dismutase). The growth of seedlings was inhibited by Ni, however, less after HBL pre-treatment. The protein content and antioxidative enzyme activities were also increased by HBL treatment.

Additional key words

antioxidative enzymes brassinosteroids heavy metal toxicity Indian mustard 



ascorbate peroxidase


bioconcentration factor






glutathione reductase




guaiacol peroxidase


reactive oxygen species


superoxide dismutase


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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • P. Sharma
    • 1
  • R. Bhardwaj
    • 1
  • N. Arora
    • 1
  • H. K. Arora
    • 1
  • A. Kumar
    • 2
  1. 1.Department of Botanical and Environmental SciencesGuru Nanak Dev University, AmritsarPunjabIndia
  2. 2.Serum Institute of India Ltd.PuneIndia

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