Biologia Plantarum

, Volume 52, Issue 4, pp 735–739 | Cite as

Assessment of genetic stability of in vitro grown Dictyospermum ovalifolium

  • M. Chandrika
  • Thoyajaksha
  • V. Ravishankar Rai
  • K. Ramachandra Kini
Brief Communication
First Online:
Received:
Accepted:

Abstract

In the present study, a polymerase chain reaction (PCR)-based method namely inter simple sequence repeat (ISSR) was employed to assess genetic stability in tissue culture-derived Dictyospermum ovalifolium plantlets. To study genomic stability of micropropagated plants, 14 individuals were randomly tagged among a population of 2500 regenerants and were compared with single donor mother plant. A total of 51 clear and reproducible bands ranging from 200 bp to 2.1 kb were scored corresponding to an average of 3.64 bands per primer. Two of the 51 bands were polymorphic (3.92 %) among 14 individuals, thus indicating the occurrence of low level genomic variation in the micropropagated plants. Cluster analysis indicates that genetic similarity values were 0.978 which allows classification of the plants to distinct groups. Further an attempt was made to reintroduce the micropropagated plants into their natural habitat. Over one thousand six hundred fifty plants were successfully established.

Additional key words

ISSR molecular marker PCR micropropagation reintroduction conservation 

Abbreviations

ISSR

inter simple sequence repeat

PCR

polymerase chain reaction

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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • M. Chandrika
    • 1
  • Thoyajaksha
    • 1
  • V. Ravishankar Rai
    • 1
  • K. Ramachandra Kini
    • 1
  1. 1.Department of Studies in Applied Botany and BiotechnologyUniversity of MysoreMysoreIndia

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