Abstract
Rapid shoot multiplication of Nyctanthes arbor-tristis L. was achieved from axillary meristems on Murashige and Skoog (MS) basal medium supplemented with 1.0–1.5 mg dm−3 6-benzylaminopurine (BA), 50 mg dm−3 adenine sulfate (Ads) and 3 % (m/v) sucrose. Inclusion of indole-3-acetic acid (IAA) in the culture medium along with BA + Ads promoted a higher rate of shoot multiplication. Maximum mean number of microshoots per explant (6.65) was achieved on the MS medium supplemented with 1.5 mg dm−3 BA, 50 mg dm−3 Ads and 0.1 mg dm−3 IAA after 4 weeks of culture. The elongated shoots rooted within 13 to 14 d on half-strength MS medium supplemented with either indole-3-butyric acid (IBA), IAA or 1-naphthaleneacetic acid (NAA) with 2 % sucrose. Maximum percentage of rooting was obtained on medium having 0.25 mg dm−3 IBA and 0.1 mg dm−3 IAA. About 70 % of the rooted plantlets survived in the greenhouse. The in vitro raised plants were grown normally in the field.
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Abbreviations
- Ads:
-
adenine sulfate
- BA:
-
6-benzylaminopurine
- IAA:
-
indole-3-acetic acid
- IBA:
-
indole-3-butyric acid
- MS medium:
-
Murashige and Skoog medium
- NAA:
-
1-naphthaleneacetic acid
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Rout, G.R., Mahato, A. & Senapati, S.K. In vitro clonal propagation of Nyctanthes arbor-tristis . Biol Plant 52, 521–524 (2008). https://doi.org/10.1007/s10535-008-0101-9
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DOI: https://doi.org/10.1007/s10535-008-0101-9