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Biologia Plantarum

, 52:413 | Cite as

Production of reactive oxygen species and development of antioxidative systems during in vitro growth and ex vitro transfer

  • P. Baťková
  • J. Pospíšilová
  • H. Synková
Review

Abstract

Ex vitro transfer is often stressful for in vitro grown plantlets. Water stress and photoinhibition, often accompanying the acclimatization of in vitro grown plantlets to ex vitro conditions, are probably the main factors promoting production of reactive oxygen species (ROS) and in consequence oxidative stress. The extent of the damaging effects of ROS depends on the effectiveness of the antioxidative systems which include low molecular mass antioxidants (ascorbate, glutathione, tocopherols, carotenoids, phenols) and antioxidative enzymes (superoxide dismutase, ascorbate peroxidase, catalase, glutathione reductase, monodehydroascorbate reductase, dehydroascorbate reductase). This review is focused on ROS production and development of antioxidative system during in vitro growth and their further changes during ex vitro transfer.

Additional key words

ascorbate ascorbate peroxidase carotenoids catalase dehydroascorbate reductase glutathione glutathione reductase malondialdehyde peroxidase superoxide dismutase 

Abbreviations

ABA

abscisic acid

APX

ascorbate peroxidase (EC 1.11.1.11)

Car

carotenoids

CAT

catalase

DHAR

dehydroascorbate reductase (EC 1.8.5.1)

GA3

gibberellic acid

GR

glutathione reductase (EC 1.6.4.2)

LOX

lipoxygenase (EC 1.13.11.12)

MDA

malondialdehyde

MDHAR

monodehydroascorbate reductase (EC 1.6.5.4)

PEG

polyethylene glycol

POX

peroxidase (EC 1.11.1.7)

ROS

reactive oxygen species

SA

salicylic acid

SOD

superoxide dismutase (EC 1.15.1.1)

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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  1. 1.Institute of Experimental BotanyAcademy of Sciences of the Czech RepublicPragueCzech Republic
  2. 2.Faculty of ScienceCharles University PraguePragueCzech Republic

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