Biologia Plantarum

, Volume 52, Issue 2, pp 391–395 | Cite as

Effect of wounding on chalcone synthase and pathogenesis related PR-10 gene expression and content of phenolic compounds in bilberry leaves

  • L. Jaakola
  • J. J. Koskimäki
  • K. R. Riihinen
  • A. Tolvanen
  • A. Hohtola
Brief Communication
Received:
Accepted:

Abstract

The influence of artificial wounding on biosynthesis of flavonoids and hydroxycinnamic acids was studied in bilberry leaves using two separate wounding experiments. In the first experiment bilberry leaves were wounded by cutting. The expression of the first gene from flavonoid pathway, chalcone synthase (CHS) and a wound induced pathogenesis related PR-10 gene was analysed from samples collected immediately and after 3, 6, 24 h and 4 d from the wounding treatment. In the second experiment annual shoots were removed. Proanthocyanidins, flavonol glycosides and hydroxycinnamic acids were quantified in leaf samples after 0–5 d (experiment 1) and 5 weeks (experiment 2) from the treatment. In the first experiment, no change was observed in the expression of CHS whereas increase in expression of PR-10 gene was detected after 6 h of wounding treatment. In both experiments, the contents of flavonol glycosides and hydroxycinnamic acids were not influenced by the wounding treatment and the contents of proanthocyanidins were decreased.

Additional key words

flavonoids hydroxycinnamic acids simulated herbivory 

Abbreviations

CTAB

hexadecyltrimethylammonium bromide

CHS

chalcone synthase

DAD

diode-array detection

GAPDH

glyceraldehyde-3-phosphate dehydrogenase

PR-10

pathogenesis related protein 10

This is a preview of subscription content, log in to check access.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Bernards, M.A., Lewis, N.G.: Alkyl ferulates in wound healing potato tubers.-Phytochemistry 31: 3409–3412, 1992.PubMedCrossRefGoogle Scholar
  2. Bowles, D.J.: Defence-related proteins in higher plants.-Annu. Rev. Biochem. 59:873–907, 1990.PubMedCrossRefGoogle Scholar
  3. Briggs, M.A.: Relation of Spodoptera eridania choice to tannins and protein of Lotus corniculatus.-J. chem. Ecol. 16: 1557–1564, 1990.CrossRefGoogle Scholar
  4. De Bruxelles, G.L., Roberts, M.R.: Signals regulating multiple responses to wounding and herbivores.-Crit. Rev. Plant Sci. 20: 487–521, 2001.CrossRefGoogle Scholar
  5. Ceballos, L., Andary, C., Delescluse, M., Gibernau, M., McKey, D., Hossart-McKey, M.: Effects of sublethal attack by a suckling insect, Hyalymenus tarsatus, on Sesbania drummondii seeds: impact on some seed traits related to fitness.-EcoScience 9: 28–36, 2002.Google Scholar
  6. Dixon, R.A., Paiva, N.L.: Stress-induced phenylpropanoid metabolism.-Plant Cell 7: 1085–1097, 1995.PubMedCrossRefGoogle Scholar
  7. Dixon, R.A., Xie, D., Sharma, S.B.: Proanthocyanidins — a final frontier in flavonoid research?-New Phytol. 165: 9–28, 2005.PubMedCrossRefGoogle Scholar
  8. Fernández-Calvo, I.C., Obeso, J.R.: Growth, nutrient content, fruit production and herbivory in bilberry Vaccinium myrtillus L. along an altitudinal gradient.-Forestry 77: 213–223, 2004.CrossRefGoogle Scholar
  9. Graham, M.Y., Weidner, J., Wheeler, K., Pelow, M.J., Graham, T.L.: Induced expression of pathogenesis-related protein genes in soybean by wounding and the Phytophthora sojae cell wall glucan elicitor.-Physiol. mol. Plant. Pathol. 63: 141–149, 2003.CrossRefGoogle Scholar
  10. Harborne, J.B., Williams, C.: Advances in flavonoid research since 1992.-Phytochemistry 55: 481–504, 2000.PubMedCrossRefGoogle Scholar
  11. Haukioja, E.: Putting the insect into birch-insect interaction.-Oecologia 136: 161–168, 2003.PubMedCrossRefGoogle Scholar
  12. Housti, F., Andary, C., Gargadennec, A., Amssa, M., Effects of wounding and salicylic acids on hydroxycinnamoylmalic acids in Thunbergia alata.-Plant Physiol. Biochem. 40: 761–769, 2002.CrossRefGoogle Scholar
  13. Jaakola, L., Määttä, K., Pirttilä, A.M., Törrönen, R., Kärenlampi, S., Hohtola, A.: Expression of structural genes involved in anthocyanin biosynthesis in relation to anthocyanin and flavonoid levels during bilberry (Vaccinium myrtillus L.) fruit development.-Plant Physiol. 130: 729–739, 2002.PubMedCrossRefGoogle Scholar
  14. Jaakola, L., Määttä-Riihinen, K., Kärenlampi, S., Hohtola, A.: Activation of flavonoid biosynthesis by solar radiation in bilberry (Vaccinium myrtillus L.) leaves.-Planta 218: 721–728, 2004.PubMedCrossRefGoogle Scholar
  15. Jaakola, L., Pirttilä, A.M., Halonen, M., Hohtola, A.: Isolation of high quality RNA from the bilberry (Vaccinium myrtillus L.) fruit.-Mol. Biotechnol. 19: 201–203, 2001b.PubMedCrossRefGoogle Scholar
  16. Jaakola, L., Pirttilä, A.M., Hohtola, A.: cDNA blotting offers an alternative method for gene expression studies.-Plant mol. Biol. Rep. 19: 125–128, 2001c.CrossRefGoogle Scholar
  17. Jaakola, L., Tolvanen, A., Laine, K., Hohtola, A.: Effect of N6-isopentenyladenine concentration on growth initiation in vitro and rooting of bilberry and lingonberry microshoots.-Plant Cell Tissue Organ Cult. 66: 73–77, 2001a.CrossRefGoogle Scholar
  18. Laine, K., Henttonen, H.: Phenolics/nitrogen ratios in the blueberry Vaccinium myrtillus in relation to temperature and microtine density in Finnish Lapland.-Oikos 50: 389–395, 1987.CrossRefGoogle Scholar
  19. Liu, J.J., Ekramoddoullah, A.K.M., Yu, X: Differential expression of multiple PR10 proteins in western white pine following wounding, fungal infection and cold hardening.-Physiol. Plant. 119: 544–553, 2003.CrossRefGoogle Scholar
  20. Lawton, M.A., Lamb, C.J.: Transcriptional activation of plant defence genes by jmcv fungal elicitor, wounding, and infection.-Mol. cell. Biol. 7: 335–341, 1987.PubMedGoogle Scholar
  21. Marles, M.A.S., Ray, H., Gruber, M.Y. New perspectives on proanthocyanidin biochemistry and molecular regulation.-Phytochemistry 64: 357–383, 2003.CrossRefGoogle Scholar
  22. Määttä, K., Kamal-Eldin, A., Törrönen, R. Phenolic compounds in berries of black, red, green, and white currants (Ribes sp.).-Antioxid. Redox Signaling 3: 981–993, 2001.CrossRefGoogle Scholar
  23. Pinto, M.P., Ribeiro, A., Regalado, A.P., Rodrigues-Pousada C., Ricardo C.P.P.: Expression of Lupinus albus PR-10 proteins during root and leaf development.-Biol. Plant. 49: 187–193, 2005.CrossRefGoogle Scholar
  24. Richard, S., Lapointe, G., Rutledge, R.G., Séguin, A.: Induction of chalcone synthase expression in white spruce by wounding and jasmonate.-Plant Cell. Physiol. 41: 982–987, 2000.PubMedCrossRefGoogle Scholar
  25. Roda, A.L., Oldham, N.J., Svatos, A., Baldwin, I.T.: Allometric analysis of the induced flavonols on the leaf surface of wild tobacco (Nicotiana attenuata).-Phytochemistry 62: 527–536, 2003.PubMedCrossRefGoogle Scholar
  26. Sallaud, C., El-Turk, J., Breda, C., Buffard, D., De Kozak, I., Esnault, R., Kondorosi, A.: Differential expression of cDNA coding for chalcone reductase, a key enzyme of the 5-deoxyflavonoid pathway, under various stress conditions in Medicago sativa.-Plant Sci. 109: 179–190, 1995.CrossRefGoogle Scholar
  27. Selas, V.: Population dynamics of capercaille Tetrao urogallus in relation to bilberry Vaccinium myrtillus production in southern Norway.-Wildlife Biol. 6: 1–11, 2000.Google Scholar
  28. Selas, V.: Autumn population size of capercaille Tetrao urogallus in relation to bilberry Vaccinium myrtillus production and weather: an analysis of Norwegian game reports.-Wildlife Biol. 7: 17–25, 2001.Google Scholar
  29. Tolvanen, A.: Differences in recovery between a deciduous and an evergreen ericaceous clonal dwarf shrub after simulated aboveground herbivory and belowground damage.-Can. J. Bot. 72: 853–859, 1994.CrossRefGoogle Scholar
  30. Tolvanen, A., Laine, K.: Effects of reproduction and artificial herbivory on vegetative growth and resource levels in deciduous and evergreen dwarf shrubs.-Can. J. Bot. 75: 656–666, 1997.CrossRefGoogle Scholar
  31. Vogt, T., Pollak, P., Tarlyn, N., Taylor, L.P.: Pollination-or wound-induced kaempferol accumulation in petunia stigmas enhances seed production.-Plant Cell 6: 11–23, 1994.PubMedCrossRefGoogle Scholar
  32. Witzell, J., Gref, R., Näsholm, T.: Plant-part specific and temporal variation in phenolic compounds of boreal bilberry (Vaccinium myrtillus) plants.-Biochem. System. Ecol. 31: 115–127, 2003.CrossRefGoogle Scholar

Copyright information

© Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Praha 2008

Authors and Affiliations

  • L. Jaakola
    • 1
  • J. J. Koskimäki
    • 1
  • K. R. Riihinen
    • 2
  • A. Tolvanen
    • 3
  • A. Hohtola
    • 1
  1. 1.Department of BiologyUniversity of OuluOuluFinland
  2. 2.Department of BiosciencesUniversity of KuopioKuopioFinland
  3. 3.Finnish Forest Research InstituteMuhos Research StationMuhosFinland

Personalised recommendations