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Biologia Plantarum

, Volume 52, Issue 2, pp 334–338 | Cite as

Direct shoot regeneration from leaf explants of Spilanthes acmella

  • K. V. Saritha
  • C. V. NaiduEmail author
Brief Communication

Abstract

Multiple shoots of Spilanthes acmella Murr. were induced from nodal buds of in vivo and in vitro seedlings on Murashige and Skoog (MS) medium containing 1.0 mg dm−3 6-benzyladenine (BA) and 0.1 mg dm−3 α-naphthalene-acetic acid (NAA). Adventitious shoots were successfully regenerated from the leaf explants derived from the above mentioned multiple shoots. The efficiency of shoot regeneration was tested in the MS medium containing BA, kinetin, or 2-isopentenyl adenine in combination with NAA, indole-3-acetic acid (IAA), or indole-3-butyric acid (IBA) and gibberellic acid. Maximum number of shoots per explant (20 ± 0.47) was recorded with 3.0 mg dm−3 BA and 1.0 mg dm−3 IAA. An anatomical study confirmed shoot regeneration via direct organogenesis. About 95 % of the in vitro shoots developed roots after transfer to half strength MS medium containing 1.0 mg dm−3 IBA. 95 % of the plantlets were successfully acclimatized and established in soil. The transplanted plantlets showed normal flowering without any morphological variation.

Additional key words

growth regulators medicinal plant tissue culture 

Abbreviations

BA

6-benzyladenine

GA3

gibberelic acid

IAA

indole-3-acetic acid

IBA

indole-3-butyric acid

2-iP

2-isopentenyl adenine (2-γ,γ-dimethylallyl aminopurine)

Kn

kinetin

NAA

α-naphthalene acetic acid

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Copyright information

© Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Praha 2008

Authors and Affiliations

  1. 1.Plant Biotechnology Division, Department of BiotechnologySri Venkateswara University, TirupatiAndhra PradeshIndia

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