Biologia Plantarum

, Volume 52, Issue 1, pp 161–164 | Cite as

Water stress induced changes in the leaf lipid composition of four grapevine genotypes with different drought tolerance

  • I. Toumi
  • M. Gargouri
  • I. Nouairi
  • P. N. Moschou
  • A. Ben Salem-Fnayou
  • A. Mliki
  • M. Zarrouk
  • A. Ghorbel
Brief Communication

Abstract

To dissect differences in both lipid accumulation and composition and the role of these modifications during drought stress, four grapevine cultivars exhibiting differential tolerance to drought were subjected to water shortage. Tolerant cultivars, Kahli Kerkennah and Cardinal, exhibited higher leaf water potential (Ψw), and lower lipid peroxidation compared to the sensitive cultivars Guelb Sardouk and Superior Seedless during stress. Total lipid amounts increased during stress only in the leaves of the tolerant cultivars. Drought induced increases in the ratios digalactosyldiacylglycerol/monogalactosyldiacylglycerol and phosphatidylcholine/phoshatidylethanolamine of almost all the drought stressed cultivars. Moreover, the overall analysis of the composition of fatty acids revealed that a linolenic acid was prevalent in grapevine and the unsaturation level of lipids increased under water stress in all the cultivars. Specific adjustments in the lipid composition during stress could compromise stress tolerance.

Additional key words

glycolipids leaf water potential peroxidation phospholipids unsaturation Vitis vinifera 

Abbreviations

C16:0

palmitic acid

C16:1

palmitoleic acid

C16:3

palmitolenic acid

C18:0

stearic acid

C18:1

oleic acid

C18:2

linoleic acid

C18:3

linolenic acid

DBI

double bond index

DGDG

digalactosyldiacylglycerol

GL

glycolipids

MGDG

monogalactosyldiacylglycerol

PC

phosphatidylcholine

PE

phoshatidylethanolamine

PG

phosphatidylglycerol

PL

phospholipids

TLC

thin layer chromatography

Ψw

leaf water potential

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Bligh, E.G., Dyer, W.J.: A rapid method of total lipid extraction and purification.-Can. J. Biochem. Physiol. 37: 911–917, 1959.PubMedGoogle Scholar
  2. Choné, X., Van Leeuwen, C., Dubourdieu, D., Gaudillere, J.P.: Stem water potential is a sensitive indicator of grapevine water status.-Ann. Bot. 87: 477–483, 2001.CrossRefGoogle Scholar
  3. El Kahoui, S., Smaoui, A., Zarrouk, M., Ghriri, R., Limam, F.: Salt-induced lipid changes in Catharanthus roseus cultured cell suspensions.-Phytochemistry 65: 1911–1917, 2004.CrossRefGoogle Scholar
  4. Fazeli, F., Ghorbanli, M., Niknam, V.: Effect of drought on biomass, protein content, lipid peroxidation and antioxidant enzymes in two sesame cultivars.-Biol. Plant. 51: 98–103, 2007.CrossRefGoogle Scholar
  5. Gigon, A., Matos, A., Laffray, D., Zuily-Fodil, Y., Pham-Thi, A.: Effect of drought stress on lipid metabolism in the leaves of Arabidopsis thaliana (ecotype Columbia).-Ann. Bot. 94: 345–351, 2004.PubMedCrossRefGoogle Scholar
  6. Grzesiak, M.T., Grzesiak, S., Skoczowski, A.: Changes of leaf water potential and gas exchange during and after drought in triticale and maize genotypes differing in drought tolerance.-Photosynthetica 44: 561–568, 2006.CrossRefGoogle Scholar
  7. Koca, H., Ozdemir, F., Turkan, I.: Effect of salt stress on lipid peroxidation and superoxide dismutase and peroxidase activities of Lycopersicon esculentum and L. pennellii.-Biol. Plant. 50: 745–748, 2006.CrossRefGoogle Scholar
  8. Lepage, M.: Identification and composition of turnip root lipids.-Lipids 2: 244–50, 1967.PubMedCrossRefGoogle Scholar
  9. Metcalfe, D., Schmitz, A., Pelka, J.R.: Rapid preparation of fatty acid esters from lipids for gas chromatographic analysis.-Anal. Chem. 38: 524–535, 1966.CrossRefGoogle Scholar
  10. Pospíšilová, J., Vágner, M., Malbeck, J., Trávníčková, A., Bat’ková, P.: Interactions between abscisic acid and cytokinins during water stress and subsequent rehydration.-Biol. Plant. 49: 533–540, 2005.CrossRefGoogle Scholar
  11. Quartacci, M.F., Glisic, O., Steranovic, B., Navarri-Izzo, F.: Plasma membrane lipids in the resurrection plant Ramonda serbica following dehydration and rehydration.-J. exp. Bot. 53: 2159–2166, 2002.PubMedCrossRefGoogle Scholar
  12. Quartacci, M.F., Pinho, C., Sgherri, C.L.M, Navari-lzzo, F.: Lipid composition and protein dynamics in thylakoids of two wheat cultivars differently sensitive to drought.-Plant Physiol. 108: 191–197, 1995.PubMedGoogle Scholar
  13. Scholander, P.F., Hammel, H.T., Hemmingsen, E.A., Bradstreet, E.D.: Hydrostatic pressure and osmotic potential in leaves of mangroves and some other plants.-Proc. nat. Acad. Sci. USA 52: 119–125, 1964.PubMedCrossRefGoogle Scholar
  14. Schultz, H.R.: Water relations and photosynthetic responses of two grapevine cultivars of different geographical origin during water stress.-Acta Hort. 427, 1996Google Scholar
  15. Singh, S.C., Sinha, R.P., Hader, D.P.: Role of lipids and fatty acids in stress tolerance in Cyanobacteria.-Acta Protozool. 41: 297–308, 2002.Google Scholar
  16. Vassilev, A., Lidon, F., Scotti, P., Da Graca, M., Yordanov, I.: Cadmium-induced changes in chloroplast lipids and photosystem activities in barley plants.-Biol. Plant. 48: 153–156, 2004.CrossRefGoogle Scholar
  17. Webb, M., Green, B.R.: Effects of neutral and anionic lipids on digalactosyldiacylglycerol vesicle aggregation.-Biochim. biophys. Acta 1030: 231–237, 1990.PubMedCrossRefGoogle Scholar
  18. Welti, R., Weiqi, L., Maoyin, L., Yongming, S., Homigol, B., Han, E.Z., Rajashekar, C.B., Williams, T.D., Wang, X.: Profiling membrane lipids in plant stress responses: role of phospholipase D in freezing-induced lipid changes in Arabidopsis.-J. biol. Chem. 33: 31994–32002, 2002CrossRefGoogle Scholar
  19. Zhang, G., Slaski, J.J., Archambault, D.J., Taylor, G.J.: Alteration of plasma membrane lipids in aluminium-resistant and aluminium-sensitive wheat genotype in response to aluminium.-Physiol. Plant. 99: 302–308, 1997.CrossRefGoogle Scholar

Copyright information

© Institute of Experimental Botany, ASCR 2008

Authors and Affiliations

  • I. Toumi
    • 1
  • M. Gargouri
    • 1
  • I. Nouairi
    • 2
  • P. N. Moschou
    • 3
  • A. Ben Salem-Fnayou
    • 1
  • A. Mliki
    • 1
  • M. Zarrouk
    • 2
  • A. Ghorbel
    • 1
  1. 1.Laboratory of Grapevine Molecular PhysiologyHammam-LifTunisia
  2. 2.Laboratory of Characterization and Quality of Olive OilHammam-LifTunisia
  3. 3.Laboratory of Plant physiology and BiotechnologyUniversity of CreteHeraklionGreece

Personalised recommendations