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Biologia Plantarum

, Volume 52, Issue 1, pp 146–148 | Cite as

Micropropagation of Ailanthus altissima and in vitro heavy metal tolerance

  • E. Gatti
Brief Communication

Abstract

Ailanthus altissima, a fast-growing and contamination-resistant species is investigated for its use in areas contaminated by heavy metals. A micropropagation protocol for A. altissima was developed, cultured shoots were tested for in vitro heavy metals tolerance. Proliferation rate and shoot length were affected by 6-benzylaminopurine (BAP) and Murashige and Skoog’s (MS) salt concentrations, best results were obtained in full strength MS medium supplemented with 1.32 or 2.64 µM BAP. Rooting percentage was strongly influenced by indole-3-butyric acid. Cultures of A. altissima exposed to heavy metals demonstrated a tolerance comparable to species already utilized in phytoremediation.

Additional key words

shoot culture copper zinc manganese phytoremediation 

Abbreviations

BAP

6-benzylaminopurine

IBA

indole-3-butyric acid

IAA

3-indoleacetic acid

NAA

naphthalene-1-acetic acid

MS

Murashige and Skoog’s (1962) medium

SH

Shenk and Hildebrandt’s (1972) medium

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Copyright information

© Institute of Experimental Botany, ASCR 2008

Authors and Affiliations

  1. 1.Istituto Biometeorologia IBIMET-CNRSezione di BolognaBolognaItaly

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