Biologia Plantarum

, Volume 51, Issue 4, pp 647–652 | Cite as

Paclitaxel and baccatin III production induced by methyl jasmonate in free and immobilized cells of Taxus baccata

  • M. Bonfill
  • S. Bentebibel
  • E. Moyano
  • J. Palazón
  • R. M. Cusidó
  • R. Eibl
  • M. T. Piñol
Original Papers

Abstract

The effects of 100 and 200 µM methyl jasmonate (MJA) on cell proliferation and paclitaxel and baccatin III production were investigated in free and alginate immobilized cells of Taxus baccata growing in a selected product formation culture medium. The greatest accumulation of paclitaxel (13.20 mg dm−3) and baccatin III (4.62 mg dm−3) occurred when 100 µM MJA was added to the culture medium of cells entrapped using a 1.5 and 2.5 % alginate solution. The effects of different treatments on the viability of cultured cells and their capacity to excrete both taxanes into the surrounding medium were considered.

Additional key words

alginate entrapped cells cell cultures elicitation taxanes yew 

Abbreviations

B5

Gamborg’s B5 medium

BAP

benzylaminopurine

MJA

methyl jasmonate

NAA

naphthaleneacetic acid

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Copyright information

© Institute of Experimental Botany, ASCR 2007

Authors and Affiliations

  • M. Bonfill
    • 1
  • S. Bentebibel
    • 1
  • E. Moyano
    • 2
  • J. Palazón
    • 1
  • R. M. Cusidó
    • 1
  • R. Eibl
    • 3
  • M. T. Piñol
    • 1
  1. 1.Laboratorio de Fisiologia Vegetal, Facultad de FarmaciaUniversidad de BarcelonaBarcelonaSpain
  2. 2.Departament de Ciències Experimentals i de SalutUniversitat Pompeu FabraBarcelonaSpain
  3. 3.Hochschule WädenswillWädenswillSwitzerland

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