Biologia Plantarum

, Volume 51, Issue 3, pp 593–596 | Cite as

Photosynthetic gene expression in black willow under various soil moisture regimes

Brief Communication

Abstract

This study was the first attempt to extract RNA from black willow (Salix nigra Marshall) that contains numerous secondary products and to examine the photosynthetic gene expression of black willow under a wide range of soil moisture regimes. Black willow cuttings were grown under control, continuous flooding, periodic flooding and periodic drought for 42 d. A modified lithium chloride precipitation method was used for RNA extraction. Results of real-time polymerase chain reaction showed reduced gene expression of oxygen evolving complex, large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenasse and ferredoxin on day 7 as well as the latter two on day 14 in response to flooding. Therefore, decreased expression of these three genes may have contributed to the observed reduced photosynthetic capacity in response to flooding.

Additional key words

ferredoxin large subunit of Rubisco oxygen evolving complex Salix nigra 

Abbreviations

C

control

CF

continuous flooding

Fd

ferredoxin

OEC

oxygen evolving complex

PCL

phenol:choroform:isoamyl alcohol

PD

periodic drought

PF

periodic flooding

PPFD

photosynthetic photon flux density

rbcL

large subunit of Rubisco

Rubisco

ribulose-1,5-bisphosphate carboxylase/oxygenasse

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Copyright information

© Institute of Experimental Botany, ASCR 2007

Authors and Affiliations

  1. 1.Department of BiologyThe University of MemphisMemphisUSA
  2. 2.W. Harry Feinstone Center for Genomic ResearchThe University of MemphisMemphisUSA
  3. 3.Wetland Biogeochemistry Institute, School of the Coast and EnvironmentLouisiana State UniversityBaton RougeUSA

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