Biologia Plantarum

, Volume 51, Issue 3, pp 593–596 | Cite as

Photosynthetic gene expression in black willow under various soil moisture regimes

Brief Communication


This study was the first attempt to extract RNA from black willow (Salix nigra Marshall) that contains numerous secondary products and to examine the photosynthetic gene expression of black willow under a wide range of soil moisture regimes. Black willow cuttings were grown under control, continuous flooding, periodic flooding and periodic drought for 42 d. A modified lithium chloride precipitation method was used for RNA extraction. Results of real-time polymerase chain reaction showed reduced gene expression of oxygen evolving complex, large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenasse and ferredoxin on day 7 as well as the latter two on day 14 in response to flooding. Therefore, decreased expression of these three genes may have contributed to the observed reduced photosynthetic capacity in response to flooding.

Additional key words

ferredoxin large subunit of Rubisco oxygen evolving complex Salix nigra 





continuous flooding




oxygen evolving complex


phenol:choroform:isoamyl alcohol


periodic drought


periodic flooding


photosynthetic photon flux density


large subunit of Rubisco


ribulose-1,5-bisphosphate carboxylase/oxygenasse


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Bray, E.A.: Classification of genes differentially expressed during water-deficit stress in Arabidopsis thaliana: an analysis using microarray and differential expression data.-Ann. Bot. 89: 803–811, 2002.PubMedCrossRefGoogle Scholar
  2. Cheng, S.-H., Moore, B.D., Seemann, J.R.: Effects of short-and long-term elevated CO2 on the expression of ribulose-1, 5-bisphosphate carboxylase/oxygenase genes and carbohydrate accumulation in leaves of Arabidopsis thaliana (L.) Heynh.-Plant Physiol. 116: 715–723, 1998.PubMedCrossRefGoogle Scholar
  3. Cherian, S., Reddy, M.P., Ferreira, R.B.: Transgenic plants with improved dehydration-stress tolerance: progress and future prospects.-Biol. Plant. 50: 481–495, 2006.CrossRefGoogle Scholar
  4. Dennis, E.S., Dolferus, R., Ellis, M., Rahman, M., Wu, Y., Hoeren, F.U., Grover, A., Ismond, K.P., Good, A.G., Peacock, W.J.: Molecular strategies for improving waterlogging tolerance in plants.-J. exp. Bot. 51: 89–97, 2000.PubMedCrossRefGoogle Scholar
  5. Henmi, T., Miyao, M., Yamamoto, Y.: Release and reactiveoxygen-mediated damage of the oxygen-evolving complex subunits of PSII during photoinhibition.-Plant Cell Physiol. 45: 243–250, 2004.PubMedCrossRefGoogle Scholar
  6. Li, S., Pezeshki, S.R., Goodwin, S., Shields, F.D., Jr.: Physiological responses of black willow (Salix nigra) cuttings to a range of soil moisture regimes.-Photosynthetica 42: 585–590, 2004.CrossRefGoogle Scholar
  7. Liu, W.-J., Yuan, S., Zhang, N.-H., Lei, T., Duan, H.-G., Liang, H.-G., Lin, H.-H.: Effect of water stress on photosystem 2 in two wheat cultuvars.-Biol. Plant. 50: 597–602, 2006.CrossRefGoogle Scholar
  8. Mitsch, W.J., Gosselink, J.G. (ed.): Wetlands. 2nd Edition.-Van Nostrand Reinhold, New York 1993.Google Scholar
  9. Palo, R.T.: Distribution of birch (Betula spp.), willow (Salix spp.), and poplar (Populus spp.) secondary metabolites and their potential role as chemical defense against herbivores.-J. chem. Ecol. 10: 499–520, 1984.CrossRefGoogle Scholar
  10. Pezeshki, S.R., Anderson, P.H., Shields, F.D., Jr.: Effects of soil moisture regimes on growth and survival of black willow (Salix nigra) posts (cuttings).-Wetlands 18: 460–470, 1998.CrossRefGoogle Scholar
  11. Sachs, M.M., Freeling, M., Okimoto, R.: The anaerobic proteins of maize.-Cell 20: 761–767, 1980.PubMedCrossRefGoogle Scholar
  12. Vu, J.C.V., Gesch, R.W., Allen, L.H., Boote, K.J., Bowes, G.: CO2 enrichment delays a rapid, drought-induced decrease in Rubisco small subunit transcript abundance.-J. Plant Physiol. 155: 139–142, 1999.Google Scholar
  13. Williams, J., Bulman, M.P., Neill, S.J.: Wilt-induced ABA biosynthesis, gene-expression and down-regulation of rbcS messenger-RNA levels in Arabidopsis thaliana.-Physiol. Plant. 91: 177–182, 1994.CrossRefGoogle Scholar

Copyright information

© Institute of Experimental Botany, ASCR 2007

Authors and Affiliations

  1. 1.Department of BiologyThe University of MemphisMemphisUSA
  2. 2.W. Harry Feinstone Center for Genomic ResearchThe University of MemphisMemphisUSA
  3. 3.Wetland Biogeochemistry Institute, School of the Coast and EnvironmentLouisiana State UniversityBaton RougeUSA

Personalised recommendations