Photosynthetic gene expression in black willow under various soil moisture regimes
This study was the first attempt to extract RNA from black willow (Salix nigra Marshall) that contains numerous secondary products and to examine the photosynthetic gene expression of black willow under a wide range of soil moisture regimes. Black willow cuttings were grown under control, continuous flooding, periodic flooding and periodic drought for 42 d. A modified lithium chloride precipitation method was used for RNA extraction. Results of real-time polymerase chain reaction showed reduced gene expression of oxygen evolving complex, large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenasse and ferredoxin on day 7 as well as the latter two on day 14 in response to flooding. Therefore, decreased expression of these three genes may have contributed to the observed reduced photosynthetic capacity in response to flooding.
Additional key wordsferredoxin large subunit of Rubisco oxygen evolving complex Salix nigra
oxygen evolving complex
photosynthetic photon flux density
large subunit of Rubisco
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- Cheng, S.-H., Moore, B.D., Seemann, J.R.: Effects of short-and long-term elevated CO2 on the expression of ribulose-1, 5-bisphosphate carboxylase/oxygenase genes and carbohydrate accumulation in leaves of Arabidopsis thaliana (L.) Heynh.-Plant Physiol. 116: 715–723, 1998.PubMedCrossRefGoogle Scholar
- Mitsch, W.J., Gosselink, J.G. (ed.): Wetlands. 2nd Edition.-Van Nostrand Reinhold, New York 1993.Google Scholar
- Vu, J.C.V., Gesch, R.W., Allen, L.H., Boote, K.J., Bowes, G.: CO2 enrichment delays a rapid, drought-induced decrease in Rubisco small subunit transcript abundance.-J. Plant Physiol. 155: 139–142, 1999.Google Scholar