Biologia Plantarum

, Volume 51, Issue 1, pp 161–164

Withanolide production by in vitro cultures of Withania somnifera and its association with differentiation

  • M. Sharada
  • A. Ahuja
  • K. A. Suri
  • S. P. Vij
  • R. K. Khajuria
  • V. Verma
  • A. Kumar
Brief Communication

DOI: 10.1007/s10535-007-0031-y

Cite this article as:
Sharada, M., Ahuja, A., Suri, K.A. et al. Biol Plant (2007) 51: 161. doi:10.1007/s10535-007-0031-y

Abstract

Withanolides-steroidal lactones, isolated from various Solanaceous plants have received considerable attention due to their potential biological activities. Five selected withanolides (withanone, withaferin A, withanolide A, withanolide B, withanolide E) were identified by HPLC-UV (DAD) — positive ion electrospray ionization mass spectroscopy in Withania somnifera (L.) Dunal cv. WSR plants and tissues cultured in vitro at different developmental phases. Cultures were established from five explants on Murashige and Skoog’s medium supplemented with different plant growth regulators. Results suggest that production of withanolides is closely associated with morphological differentiation.

Additional key words

callus culture plant tissue culture Solanaceae steroidal lactones 

Abbreviations

B5

Gamborg’s (1968) medium

BAP

6-benzylaminopurine

2,4-D

2,4-dichlorophenoxyacetic acid

HPLC

high pressure liquid chromatography

IAA

indole-3-acetic acid

IBA

indole-3-butyric acid

KN

kinetin

MS medium

Murashige and Skoog (1962) medium

NAA

α-naphthalene acetic acid

NMR

nuclear magnetic resonance

TLC

thin layer chromatography

Copyright information

© Institute of Experimental Botany, ASCR 2007

Authors and Affiliations

  • M. Sharada
    • 1
  • A. Ahuja
    • 1
  • K. A. Suri
    • 1
  • S. P. Vij
    • 2
  • R. K. Khajuria
    • 1
  • V. Verma
    • 1
  • A. Kumar
    • 1
  1. 1.Regional Research LaboratoryCSIRJammu-TawiIndia
  2. 2.Shoolini Institute of Life Sciences & Business ManagementAnand CampusSolanIndia

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