Biologia Plantarum

, Volume 51, Issue 1, pp 157–160

Increased antioxidant activity in Cassia seedlings under UV-B radiation

  • S. Agarwal
Brief Communication

DOI: 10.1007/s10535-007-0030-z

Cite this article as:
Agarwal, S. Biol Plant (2007) 51: 157. doi:10.1007/s10535-007-0030-z

Abstract

Cassia auriculata L. seedlings were irradiated with ultraviolet B (UV-B) in an environment-control chamber. The two doses assayed (7.5 and 15.0 kJ m−2) induced oxidative damage with an increase in lipid peroxidation and hydrogen peroxide and a decrease in chlorophyll and total phenol contents. The ascorbate and dehydroascorbate content as well as the reduced glutathione/oxidized glutathione content and ratio were significantly increased. The UV-B stress led to significant increases of the activity of superoxide dismutase, catalase, peroxidase and polyphenol oxidase. It is suggested that Cassia seedlings try to counteract high concentrations of oxygen species produced under UV-B stress through a co-ordinated increase in the contents and activities of antioxidants involved in their detoxification.

Additional key words

ascorbate catalase glutathione hydrogen peroxide oxidative stress peroxidase phenol polyphenol oxidase superoxide dismutase thiobarbituric acid reactive substances 

Abbreviations

ASA

ascorbate

CAT

catalase

DHA

dehydroascorbate

DTNB

5,5′-dithio-bis-(2-nitrobenzoic acid)

EDTA

ethylenediaminetetraacetic acid (disodium salt)

f.m.

fresh mass

GSH

reduced glutathione

GSSG

oxidized glutathione

NADPH

reduced nicotinamide adenine dinucleotide phosphate

NBT

nitroblue tetrazolium

POX

peroxidase

PPO

polyphenol oxidase

PVP

polyvinylpyrrolidone

ROS

reactive oxygen substances

SOD

superoxide dismutase

TBA

thiobarbituric acid

TBARS

thiobarbituric acid reactive substances

Copyright information

© Institute of Experimental Botany, ASCR 2007

Authors and Affiliations

  • S. Agarwal
    • 1
  1. 1.Stress Physiology Laboratory, Department of BotanyJNV UniversityJodhpur, RajasthanIndia

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