Advertisement

Biologia Plantarum

, Volume 50, Issue 4, pp 745–748 | Cite as

Effect of salt stress on lipid peroxidation and superoxide dismutase and peroxidase activities of Lycopersicon esculentum and L. pennellii

  • H. Koca
  • F. Ozdemir
  • I. Turkan
Brief Communication

Abstract

In this study, a relationship between lipid peroxidation, the antioxidant defense system and salt stress in salt-sensitive cultivated tomato (Lycopersicon esculentum) and its salt-tolerant wild relative (L. pennellii) was established. Superoxide dismutase (SOD) activities were significantly higher in the leaves of L. pennellii than those of L. esculentum after 12 and 84 d. POX activity showed a gradual increase in both cultivars under 70 mM NaCl. POX activity in L. pennellii significantly increased after 6 and 84 d whereas showed no remarkable change in leaves of L. esculentum under 140 mM NaCl. A higher salinity tolerance of L. pennellii was also correlated with a lower lipid peroxidation, which might be due to a higher content of antioxidant enzymes studied.

Additional key words

antioxidant enzymes ascorbate peroxidase glutathione reductase malondialdehyde NaCl tomato 

Abbreviations

APOX

ascorbate peroxidase

BSA

bovine serum albumine

DAB

diaminobenzidine-tetrahydrochloride dehydrate

EDTA

ethylenediamine tetraacetic acid

GR

glutathione reductase

MDA

malondialdehyde

NBT

nitroblue tetrazolium

POX

peroxidase

PVPP

polyvinylpolypyrrolidone

ROS

reactive oxygen species

SOD

superoxide dismutase

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Agarwal, S., Pandey, V.: Antioxidant enzyme responses to NaCl stress in Cassia angustifolia.-Biol. Plant. 48: 555–560, 2004.CrossRefGoogle Scholar
  2. Beauchamp, C., Fridovich, I.: Superoxide dismutase: improved assays and an assay applicable to acrylamide gels.-Anal. Biochem. 44: 276–287, 1971.PubMedCrossRefGoogle Scholar
  3. Bolarin, M.C., Fernandez, F.G., Cruz, V., Cuartero, J.: Salinity tolerance in four wild tomato species using vegetative yield-salinity responses curves.-Amer. Soc. hort. Sci. 116: 266–290, 1991.Google Scholar
  4. Bor, M., Özdemir, F., Türkan, İ.: The effect of salt stres on lipid peroxidation and antioxidants in leaves of sugar beet Beta vulgaris L. and wild beet Beta maritima L.-Plant Sci. 164: 77–84, 2003.CrossRefGoogle Scholar
  5. Bradford, M.M: A rapid and sensitive method for the quatitation of microgram quantities of protein utilizing the principle of protein-dye binding.-Anal. Biochem. 72: 248–254, 1976.PubMedCrossRefGoogle Scholar
  6. Cuartero, J., Yeo, A.R., Flowers, T.J.: Selection of donors for salt-tolerance in tomato using physiological traits.-New Phytol. 121: 63–69, 1992.CrossRefGoogle Scholar
  7. Demiral, T., Türkan, I.: Does exogenous glycinebetaine affect antioxidative system of rice seedlings under NaCl treatment?-J. Plant Physiol. 161: 1089–1100, 2004.PubMedCrossRefGoogle Scholar
  8. Ghorbanli, M., Ebrahimzadeh, H., Sharifi, M.: Effects of NaCl and mycorrhizal fungi on antioxidative enzymes in soybean.-Biol. Plant. 48: 575–581, 2004.CrossRefGoogle Scholar
  9. Herzog, V., Fahimi, H.: Determination of the activity of peroxidase.-Anal. Biochem. 55: 554–562, 1973.PubMedCrossRefGoogle Scholar
  10. Madhavara Rao, K.V., Sresty, T.V.S.: Antioxidative parameters in the seedlings of pigeonpea (Cajanus cajan (L). Millspaugh) in response to Zn and Ni stresses.-Plant Sci. 157: 113–128, 2000.CrossRefGoogle Scholar
  11. Meloni, D.A., Oliva, M.A., Martinez, C.A., Cambraia, J.: Photosynthesis and activity of superoxidase dismutase, peroxidase and glutathion reductase in cotton under salt stres.-Environ. exp. Bot. 49: 69–76, 2003.CrossRefGoogle Scholar
  12. Mittova, V., Guy, M., Tal, M., Volokita, M.: Response of the cultivated tomato and its wild salt-tolerant relative Lycopersicon pennellii to salt-dependent oxidative stress: Increased activities of antioxidant enzymes in root plastids.-Free Radical Res. 36: 195–202, 2002.CrossRefGoogle Scholar
  13. Mondal, K., Sharma, N.S., Malhotra, S.P., Dhawan, K., Singh, R.: Antioxidant systems in ripening tomato fruits.-Biol. Plant. 48: 49–53, 2004.CrossRefGoogle Scholar
  14. Rush, D.W., Epstein, E.: Breeding and selection for salt tolerance by the incorporation of wild germplasm into a domestic tomato.-J. amer. Soc. hort. Sci. 106: 699–704, 1981.Google Scholar
  15. Shalata, A., Tal, M.: The effect of salt stress on lipid peroxidation and antioxidants in the of the cultivated tomato and its wild salt-tolerant relative Lycopersicon pennellii.-Physiol. Plant. 104: 169–174, 1998.CrossRefGoogle Scholar

Copyright information

© Institute of Experimental Botany, ASCR, Praha 2006

Authors and Affiliations

  1. 1.Department of Biology, Science FacultyEge University, BornovaIzmirTurkey

Personalised recommendations