Biologia Plantarum

, Volume 50, Issue 4, pp 496–500 | Cite as

Micropropagation of Coleus blumei from nodal segments and shoot tips

  • G. Rani
  • D. Talwar
  • A. NagpalEmail author
  • G. S. Virk
Original Paper


A rapid and highly-effective method for micropropagation from nodal segment and shoot tip explants was established for Coleus blumei Benth. Nodal segments and shoot tips were inoculated on MS medium containing 0.7 % agar, 3 % commercial sugar, and different combinations of 6-benzyladenine (BA) with indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) or α-naphthaleneacetic acid (NAA). Hundred percent shoot induction from both explants was achieved on the medium containing BA (2 mg dm−3) and NAA (1 mg dm−3). Shoot tips were proved to be the better explant in comparison to nodal segments in having high rate of shoot induction and more number of shoots. The same media conditions were found suitable for shoot multiplication. Multiplied shoots rooted best on MS medium supplemented with IBA (2 mg dm−3). Micropropagated plants were successfully established in soil after hardening, with 100 % survival rate.

Additional key words

agar concentration carbon source growth regulators shoot differentiation tissue culture 





indole-3-acetic acid


indole-3-butyric acid


α-naphthaleneacetic acid

MS medium

Murashige and Skoog’s (1962) medium


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Anand, A., Rao, C.S., Balakrishna, P.: In vitro propagation of Syzygium travancoricum Gamble-an endangered tree species.-Plant Cell Tissue Organ Cult. 56: 59–63, 1999.CrossRefGoogle Scholar
  2. Bains, P.S.: A low cost and simple medium for micropropagation of potato.-In Vitro 27: 45A, 1991.Google Scholar
  3. Bhattacharya, S., Bhattacharya, S.: Rapid multiplication of Jasminum officinale L. by in vitro culture of nodal explants.-Plant Cell Tissue Organ Cult. 51: 57–60, 1997.CrossRefGoogle Scholar
  4. Bhattacharya, R., Bhattacharya, S.: In vitro multiplication of Coleus forskohlii Briq.: an approach towards shortening the protocol.-In Vitro cell. dev. Biol. Plant 37: 572–575, 2001.CrossRefGoogle Scholar
  5. Bringmann, G., Rischer, H., Schlauer, J., Aké Assi, L.: In vitro propagation of Ancistrocladus abbreviatus Airy Shaw (Ancistrocladaceae).-Plant Cell Tissue Organ Cult. 57: 71–73, 1999.CrossRefGoogle Scholar
  6. Echeverrigaray, S., Basso, R., Andrade, L.B.: Micropropagation of Lavandula dentata from axillary buds of field-grown adult plants.-Biol. Plant. 49: 439–442, 2005.CrossRefGoogle Scholar
  7. George, P.S., Ravishankar, G.A.: In vitro multiplication of Vanilla planifolia using axillary bud explants.-Plant Cell Rep. 16: 490–494, 1997.Google Scholar
  8. Hiregoudar, L.V., Kumar, H.G.A., Murthy, H.N.: In vitro culture of Feronia limonia (L.) Swingle from hypocotyls and internodal explants.-Biol. Plant. 49: 41–45, 2005.CrossRefGoogle Scholar
  9. Ibrahim, K.M., Collins, J.C., Collin, H.A.: Characterization of progeny of Coleus blumei following an in vitro selection for salt tolerance.-Plant Cell Tissue Organ Cult. 28: 139–145, 1992.CrossRefGoogle Scholar
  10. Iriondo, J.M., De la Iglesia, M., Perez, C.: Micropropagation of Elaeagnus angustifolia from mature trees.-Tree Physiol. 15: 691–693, 1995.PubMedGoogle Scholar
  11. Kannan, R., Jasrai, Y.T.: Micropropagation of medicinal plant-Vitex negundo.-J. med. arom. Plant Sci. 20: 693–696, 1998.Google Scholar
  12. Manjula, S., Thomas, A., Daniel, B., Nair, G.M.: In vitro plant regeneration of Aristolochia indica through axillary shoot multiplication and organogenesis.-Plant Cell Tissue Organ Cult. 51: 145–148, 1997.CrossRefGoogle Scholar
  13. Marcotrigiano, M., Boyle, T.H., Morgan, P.A., Ambach, K.L.: Leaf color variants from Coleus shoot cultures.-J. amer. Soc. hort. Sci. 115: 681–686, 1990.Google Scholar
  14. Martin, K.P.: Rapid in vitro multiplication and ex vitro rooting of Rotula aquatica Lour., a rare rhoeophytic woody medicinal plant.-Plant Cell. Rep. 21: 415–420, 2003.PubMedGoogle Scholar
  15. Meinhard, J., Petersen, M., Alfermann, A.W.: Rosmarinic acid in organ cultures of Coleus blumei.-Planta med. 59(Suppl.): 7, 1993.Google Scholar
  16. Meyers, L.S., Grossen, N.E.: Behavioral Research, Theory, Procedure and Design.-W.H. Freeman and Co., San Francisco 1974.Google Scholar
  17. Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue culture.-Physiol. Plant. 15: 473–497, 1962.CrossRefGoogle Scholar
  18. Nayak, S., Sen, S.: Regeneration of Asparagus robustus Hort.-J. Herbs Spices med. Plants 5: 43–50, 1998.CrossRefGoogle Scholar
  19. Petersen, M., Szabo, E., Meinhard, J., Karwatzki, B., Gertlowski, C.: Biosynthesis and accumulation of rosmarinic acid in suspension cultures of Coleus blumei.-Plant Cell Tissue Organ Cult. 43: 89–92, 1995.CrossRefGoogle Scholar
  20. Ponsamuel, J., Samson, N.P., Anderson, K.P.: In vitro clonal propagation of country potato-an underexploited tuber plant.-In Vitro 30: A71, 1994.Google Scholar
  21. Sen, J., Sharma, A.K.: In vitro propagation of Coleus forskohlii Briq. for forskolin synthesis.-Plant Cell Rep. 9: 696–698, 1991.CrossRefGoogle Scholar
  22. Sharma, N., Chandel, K.P.S., Srivastava, V.K.: In vitro propagation of Coleus forskohlii Briq., a threatened medicinal plant.-Plant Cell Rep. 10: 67–70, 1991.CrossRefGoogle Scholar
  23. Sharma, S.K., Ramamurthy, V.: Micropropagation of 4-year-old elite Eucalyptus tereticornis trees.-Plant Cell Rep. 19: 511–518, 2000.CrossRefGoogle Scholar
  24. Smith, R.H., Murashige, T.: Primordial leaf and phytohormone effects on excised shoot apical meristems of Coleus blumei Benth.-Amer. J. Bot. 69: 1334–1339, 1982.CrossRefGoogle Scholar
  25. Sreekumar, S., Seeni, S., Pushpangadan, P.: Micropropagation of Hemidesmus indicus for cultivation and production of 2-hydroxy-4-methoxybenzaldehyde.-Plant Cell Tissue Organ Cult. 62: 211–218, 2000.CrossRefGoogle Scholar
  26. Zagrajski, N., Leljak-Levanić, D., Jelaska, S.: Organogenesis and callogenesis in nodal, internodal and leaf explants of Coleus blumei Benth.-Periodicum Biol. 99: 67–76, 1997.Google Scholar

Copyright information

© Institute of Experimental Botany, ASCR, Praha 2006

Authors and Affiliations

  1. 1.Department of Botanical and Environmental SciencesGuru Nanak Dev UniversityAmritsarIndia

Personalised recommendations