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Biologia Plantarum

, Volume 50, Issue 4, pp 496–500 | Cite as

Micropropagation of Coleus blumei from nodal segments and shoot tips

  • G. Rani
  • D. Talwar
  • A. NagpalEmail author
  • G. S. Virk
Original Paper

Abstract

A rapid and highly-effective method for micropropagation from nodal segment and shoot tip explants was established for Coleus blumei Benth. Nodal segments and shoot tips were inoculated on MS medium containing 0.7 % agar, 3 % commercial sugar, and different combinations of 6-benzyladenine (BA) with indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) or α-naphthaleneacetic acid (NAA). Hundred percent shoot induction from both explants was achieved on the medium containing BA (2 mg dm−3) and NAA (1 mg dm−3). Shoot tips were proved to be the better explant in comparison to nodal segments in having high rate of shoot induction and more number of shoots. The same media conditions were found suitable for shoot multiplication. Multiplied shoots rooted best on MS medium supplemented with IBA (2 mg dm−3). Micropropagated plants were successfully established in soil after hardening, with 100 % survival rate.

Additional key words

agar concentration carbon source growth regulators shoot differentiation tissue culture 

Abbreviations

BA

6-benzyladenine

IAA

indole-3-acetic acid

IBA

indole-3-butyric acid

NAA

α-naphthaleneacetic acid

MS medium

Murashige and Skoog’s (1962) medium

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Copyright information

© Institute of Experimental Botany, ASCR, Praha 2006

Authors and Affiliations

  1. 1.Department of Botanical and Environmental SciencesGuru Nanak Dev UniversityAmritsarIndia

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