Advertisement

Biologia Plantarum

, Volume 50, Issue 3, pp 437–440 | Cite as

Thidiazuron induced high frequency axillary shoot multiplication in Psoralea corylifolia

  • M. Faisal
  • M. AnisEmail author
Brief Communication

Abstract

The effect of thidiazuron (TDZ) was studied on in vitro axillary shoot proliferation from nodal explant of Psoralea corylifolia - an endangered medicinal plant. Proliferation of shoots was achieved on Murashige and Skoog (MS) medium supplemented with 0.5, 1, 2, 3, 4 and 5 μM TDZ. The maximum number (13.6 ± 1.4) of shoots per explant were obtained from nodal segment cultured on 2 μM TDZ for 4 weeks and this increased to 29.7 ± 2.1 on hormone free MS medium after 8 weeks. The in vitro proliferated and elongated shoots were transferred individually on a root induction medium containing 0.5 μM indole-3-butyric acid (IBA) and within 4 weeks 4.5 ± 0.5 roots per shoot were produced. The regenerated plantlets were transferred to 1:1 soil and vermiculite mixture and acclimatized with 80 % survival rate. Fully acclimatized plants were grown in garden soil in greenhouse and their morphological and physiological parameters were comparable with seedlings.

Additional key words

ex vitro transfer indole-3-butyric acid in vitro cultivation plant regeneration 

Abbreviations

IBA

indole-3-butyric acid

MS

Murashige and Skoog medium

PFD

photon flux density

PGR

plant growth regulator

PN

net photosynthetic rate

SFC

shoot forming capacity

TDZ

thidiazuron

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Abrie, A.L., Van Staden, J.: Micropropagation of the endangered Aloe polyphylla.-Plant Growth Regul. 33: 19–23, 2001.CrossRefGoogle Scholar
  2. Bohmer, P., Meyer, B., Jacobson, H.J.: Thidiazuron induced high frequency of shoot induction and plant regeneration in protoplast derived pea callus.-Plant Cell Rep. 15: 26–29, 1995.Google Scholar
  3. Cheepala, S.B., Sharma, N.C., Sahi, S.V.: Rapid in vitro regeneration of Sesbania drummondii.-Biol. Plant. 48: 13–18, 2004.CrossRefGoogle Scholar
  4. Cocu, S., Uranbey, S., Ipek, A., Khawar, K.M., Sarihan, E.O., Kaya, M.D., Parmaksiz, I., Ozcan, S.: Adventitious shoot regeneration and micropropagation in Calendula officinalis L.-Biol. Plant. 48: 449–451, 2004.Google Scholar
  5. Faisal, M., Anis, M.: Rapid mass propagation of Tylophora indica Merrill via leaf callus culture.-Plant Cell Tissue Organ Cult. 75: 125–129, 2003.CrossRefGoogle Scholar
  6. Faisal, M., Ahmad, N., Anis, M.: Shoot multiplication in Rauvolfia tetraphylla L. using thidiazuron.-Plant Cell Tissue Organ Cult., 80: 187–190, 2005.CrossRefGoogle Scholar
  7. Fiola, J.A., Hassan, M.A., Swartz, H.J., Bors, R.H., McNicols, R.: Effect of thidiazuron, light fluence rates and kanamycin on in vitro shoot organogenesis from excised Rubus cotyledons and leaves.-Plant Cell Tissue Organ Cult. 39: 371–387, 1990.Google Scholar
  8. Huetteman, C.A., Preece, J.E.: Thidiazuron: a potent cytokinin for woody plant tissue culture.-Plant Cell Tissue Organ Cult. 33: 105–119, 1993.CrossRefGoogle Scholar
  9. Ket, N.V., Hahn, E.J., Park, S.Y., Chakarbarty, D., Paek, K.Y.: Micropropagation of an endangered orchid Anoectochilus formosanus.-Biol. Plant. 48: 339–344, 2004.CrossRefGoogle Scholar
  10. MacKinney, G.: Absorption of light by chlorophyll solution.-J. biol. Chem. 140: 315–322, 1941.Google Scholar
  11. Malik, K.A., Saxena, P.K.: Thidiazuron induces high frequency shoot regeneration in intact seedlings of Pea (Pisum sativum) and lentil (Lens culinaris).-Aust. J. Plant Physiol. 19: 731–740, 1992.Google Scholar
  12. Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassay with tobacco tissue culture.-Physiol. Plant. 15: 473–497, 1962.Google Scholar
  13. Murch, S.J., Saxena, P.K.: Molecular fate of thidiazuron and its effects on auxin transport in hypocotyls tissues of Pelargonium × hortorum Bailey.-Plant Growth Regul. 35: 269–275, 2001.CrossRefGoogle Scholar
  14. Murthy, B.N.S., Victor, J., Singh, R.P.S., Fletcher, R.A., Saxena, P.K.: In vitro regeneration of chickpea (Cicer arietinum L.) stimulation of direct organogenesis and somatic embryogenesis by thidiazuron.-Plant Growth Regul. 19: 233–240, 1996.CrossRefGoogle Scholar
  15. Saxena, C., Rout. G.R., Das, P.: Micropropagation of Psoralea corylifolia.-J. Med. Arom. Plant Sci. 20: 15–18, 1998.Google Scholar
  16. Victor, J.M.R., Murthy, B.N.S., Murch, S.J., Krishnaraj, S., Saxena, P.K.: Role of endogenous purine metabolism in thidiazuron-induced somatic embryogenesis of peanut (Arachis hypogaea L.)-Plant Growth Regul. 28: 41–47, 1999.Google Scholar

Copyright information

© Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Praha 2006

Authors and Affiliations

  1. 1.Plant Tissue Culture Laboratory, Department of BotanyAligarh Muslim UniversityAligarhIndia

Personalised recommendations