Abstract
Ferredoxin-dependent glutamate synthase (Fd-Gogat; EC 1.4.7.1) in leaf and root plastids is the last enzyme involved in the pathway of nitrate assimilation in higher plants. Arabidopsis thaliana expresses two different genes: the first, light regulated, specific of green tissues and the second expressed in other tissues. In this work, we investigated whether in our clone, OsGog2 AC Y12595, this gene is up-regulated by light or it is expressed under darkness. Fd-Gogat specific activity, protein and mRNA increased after light treatment in rice shoots. In roots, the activity and the protein content remained constant, whereas the mRNA is repressed by light treatment. The results obtained using a specific probe, situated in the 3′ untranslated region of the OsGog2 cDNA, indicated that OsGog2 gene is up-regulated by light and that its expression is tissue specific and suggested that a dark expressed Fd-Gogat gene could be present in rice similarly as in Arabidopsis.
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Abbreviations
- Fd:
-
ferredoxin
- GS:
-
glutamine synthetase
- Gogat:
-
glutamate synthase
References
Altschul, S.F., Stephen, F., Madden, T.L., Schaffer, A.A, Zhang, J., Zhang, Z., Miller, W., Lipman, D.J.: Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.-Nucl. Acids Res 25: 3389–3402, 1997.
Avila, C., Marquez, A.J., Pajuelo, P., Cannell, M.E., Wallsgrove, R.M., Forde, B.G.: Cloning and sequence analysis of a cDNA for barley ferredoxin-dependent glutamate synthase and molecular analysis of photorespiratory mutants deficient in the enzyme.-Planta 189: 475–483, 1993.
Bowsher, C.G., Boulton, E.L., Rose, J., Nayagam, S., Emes, M.J.: Reductant for glutamate synthase is generated by the oxidative pentose phosphate pathway in non-photosynthetic root plastids.-Plant J. 2: 893–898, 1992.
Coschigano, K.T., Melo-Oliveira, R., Lim, J., Coruzzi, G.M.: Arabidopsis gls mutants and distinct Fd-Gogat genes: implications for photorespiration and primary nitrogen assimilation.-Plant Cell 10: 741–752, 1998.
Garcia-Gutierrez, A., Canton, F.R., Gallardo, F., Sanchez-Jimenez, F., Ceanovas, F.M.: Expression of ferredoxin-dependent glutamate synthase in dark grown pine seedlings.-Plant mol. Biol. 27: 115–128, 1995.
Laemmli, U.K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4.-Nature 227: 680–685, 1970.
Mattana, M., Bertani, A., Aurisano, N., Reggiani, R.: Preliminary evidence of nitrate assimilation during the anaerobic germination of rice.-In: Jackson, M.B., Black, C.R. (ed.): Interacting Stresses on Plants in a Changing Climate. Pp. 365–374. Springer-Verlag, Berlin-Heidelberg-London 1993.
Mattana, M., Coraggio, I., Bertani, A., Reggiani, R.: Expression of the enzymes of nitrate reduction during the anaerobic germination of rice.-Plant Physiol. 106: 1605–1608, 1994.
Mattana, M., Coraggio, I., Brambilla, I., Bertani, A., Reggiani, R.: Nitrate assimilation during the anaerobic germination of rice: expression of ferredoxin-dependent glutamate synthase.-Planta 199: 74–78, 1996.
Mattana, M., Ida, S., Reggiani, R.: The root form of ferredoxin-NADP+ oxidoreductase is expressed in rice coleoptiles for the assimilation of nitrate.-Planta 202: 397–401, 1997.
Morigasaki, S., Takata, K., Sanada, Y., Wada, K., Yee, B.C., Shin, S., Buchanan, B.B.: Novel forms of ferredoxin and ferredoxin-NADP reductase from spinach roots.-Arch. Biochem. Biophys. 283: 75–80, 1990.
Nalbantoglu, B., Hirasawa, M., Moomaw, C., Nguyen, H., Knaff, D.B., Allen, R.: Cloning and sequencing of the gene encoding spinach ferredoxin-dependent glutamate synthase.-Biochim. biophys. Acta 1183: 557–561, 1994.
Oaks, A.: A re-evaluation of nitrogen assimilation in roots.-Bioscience 42: 103–111, 1992.
Pajuelo, P., Pajuelo, E., Forde, B.G., Marquez, A.J.: Regulation of the expression of ferredoxin-glutamate synthase in barley.-Planta 203: 517–525, 1997.
Reggiani, R., Mattana, M., Aurisano, N., Bertani, A.: Utilization of seed storage nitrate during the anaerobic germination of rice seeds.-Plant Cell Physiol. 34: 379–383, 1993.
Sakakibara, H., Watanabe, M., Hase, T.: Molecular cloning and characterization of complementary DNA encoding for ferredoxin-dependent glutamate synthase in maize leaf.-J. biol. Chem. 266: 2028–2035, 1991.
Sambrook, J., Fritsch, E.F., Maniatis, T.: Molecular Cloning: a Laboratory Manual, 2nd Ed.-Cold Spring Harbor Laboratory Press, Cold Spring Harbor 1989.
Somerville, C.R., Ogren, W.L.: Inhibition of photosynthesis in Arabidopsis mutants lacking leaf glutamate synthase activity.-Nature 286: 257–259, 1980.
Suzuki, A., Rioual, S., Lemarchand, S., Godfroy, N., Roux, Y., Boutin, J.P., Rothstein, S.: Regulation by light and metabolites of ferredoxin-dependent glutamate synthase in maize.-Physiol. Plant. 112: 524–530, 2001.
Suzuki, A., Rothstein, S.: Structure and regulation of ferredoxin-dependent glutamate synthase from Arabidopsis thaliana cloning of cDNA, expression in different tissues of wild-type and gltS mutant strains, and light induction.-Eur. J. Biochem. 243: 708–718, 1997.
Suzuki, A., Vidal, J., Gadal, P.: Glutamate isoforms in rice.-Plant Physiol. 70: 827–832, 1982.
Unnithan, S., Moraga, D.A., Schuster, S.M.: A high-performance liquid chromatography assay for asparagines synthetase.-Anal. Biochem. 136: 195–201, 1984.
Zehnacker, C., Becker, T.W., Suzuki, A., Carrayol, E., Caboche, M., Hirel, B.: Purification and properties of tobacco ferredoxin-dependentglutamate synthase, and isolation of corresponding cDNA clones.-Planta 187: 266–274, 1992.
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Mattana, M., Biazzi, E., Bertani, A. et al. Characterization of the Ferredoxin-Gogat gene (OsGog2 clone) expression in rice. Biol Plant 50, 187–192 (2006). https://doi.org/10.1007/s10535-006-0005-5
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DOI: https://doi.org/10.1007/s10535-006-0005-5