Biologia Plantarum

, Volume 50, Issue 2, pp 169–173 | Cite as

Direct somatic embryogenesis and plant regeneration from leaf explants of Phalaenopsis amabilis

  • J. T. ChenEmail author
  • W. C. Chang


Leaf explants of Phalaenopsis amabilis var. formosa formed clusters of somatic embryos directly from epidermal cells without an intervening callus within 20 – 30 d when cultured on 1/2-strength modified Murashige and Skoog medium supplemented with 0.1, 1 and 3 mg dm−3 TDZ. Repetitive production of embryos involved secondary embryogenesis could be obtained by culturing segments of embryogenic masses on TDZ-containing media. Plantlet conversion from embryos was successfully achieved on regulator-free growth medium.

Additional key words

moth orchid naphthaleneacetic acid thidiazuron 



Murashige and Skoog (1962) medium


naphthaleneacetic acid


thidiazuron (1-phenyl-3-(1,2,3-thiadiazol-5-yl)-urea


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Copyright information

© Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Praha 2006

Authors and Affiliations

  1. 1.Institute of BiotechnologyNational University of KaohsiungKaohsiungTaiwan, Republic of China
  2. 2.Department of Life SciencesNational University of KaohsiungKaohsiungTaiwan, Republic of China
  3. 3.Institute of Plant and Microbial BiologyAcademia SinicaTaipeiTaiwan, Republic of China

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