Advertisement

Biologia Plantarum

, Volume 50, Issue 2, pp 169–173 | Cite as

Direct somatic embryogenesis and plant regeneration from leaf explants of Phalaenopsis amabilis

  • J. T. ChenEmail author
  • W. C. Chang
Article

Abstract

Leaf explants of Phalaenopsis amabilis var. formosa formed clusters of somatic embryos directly from epidermal cells without an intervening callus within 20 – 30 d when cultured on 1/2-strength modified Murashige and Skoog medium supplemented with 0.1, 1 and 3 mg dm−3 TDZ. Repetitive production of embryos involved secondary embryogenesis could be obtained by culturing segments of embryogenic masses on TDZ-containing media. Plantlet conversion from embryos was successfully achieved on regulator-free growth medium.

Additional key words

moth orchid naphthaleneacetic acid thidiazuron 

Abbreviations

MS

Murashige and Skoog (1962) medium

NAA

naphthaleneacetic acid

TDZ

thidiazuron (1-phenyl-3-(1,2,3-thiadiazol-5-yl)-urea

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Arditti, J., Ernst, R. (ed.): Micropropagation of Orchids.-John Wiley & Son, New York 1993.Google Scholar
  2. Chen, J.T., Chang, C., Chang, W.C.: Direct somatic embryogenesis from leaf explants of Oncidium ‘Gower Ramsey’ and subsequent plant regeneration-Plant Cell Rep. 19: 143–149, 1999.CrossRefGoogle Scholar
  3. Chen, J.T., Chang, W.C.: Effects of auxins and cytokinins on direct somatic embryogenesis from leaf explants of Oncidium ‘Gower Ramsey’.-Plant Growth Regul. 34: 229–232, 2001.CrossRefGoogle Scholar
  4. Chen, Y., Piluek, C.: Effects of thidiazuron and N6-benzylaminopurine on shoot regeneration of Phalaenopsis.-Plant Growth Regul. 16: 99–101, 1995.Google Scholar
  5. Chen, Y.C., Chang, C., Chang, W.C.: A reliable protocol for plant regeneration from callus culture of Phalaenopsis.-In vitro cell. dev. Biol. Plant 36: 420–423, 2000.Google Scholar
  6. Duan, J.X., Chen, H., Yazawa, S.: In vitro propagation of Phalaenopsis via culture of cytokinins-induced nodes.-J. Plant Growth Regul. 15: 133–137, 1996.CrossRefGoogle Scholar
  7. Duncan, D.B.: Multiple range and multiple F test.-Biometrics 11: 1–42, 1955.Google Scholar
  8. Ernst, R.: Effects of thidiazuron on in vitro propagation of Phalaenopsis and Doritaenopsis (Orchidaceae).-Plant Cell Tissue Organ Cult. 39: 273–275, 1994.CrossRefGoogle Scholar
  9. Ishii, Y., Takamura, T., Goi, M., Tanaka, M.: Callus induction and somatic embryogenesis of Phalaenopsis.-Plant Cell Rep. 17: 446–450, 1998.CrossRefGoogle Scholar
  10. Islam, M.O., Ichihashi, S.: Effects of sucrose, maltose and sorbitol on callus growth of Phalaenopsis, Doritaenopsis and Neofinetia.-J. jap. Soc. hort. Sci. 68: 1124–1131, 1999.Google Scholar
  11. Jensen, W.A.: Botanical Histochemistry.-Freeman, San Francisco 1962.Google Scholar
  12. Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures.-Physiol. Plant. 15: 495–497, 1962.Google Scholar
  13. Park, S.Y., Murthy, H.N., Paek, K.Y.: Rapid propagation of Phalaenopsis from floral stalk-derived leaves.-In Vitro cell. dev. Biol. Plant 38: 168–172, 2002.Google Scholar
  14. Su, Y.J., Chen, J.T., Chang, W.C.: Efficient and repetitive production of leaf-derived somatic embryos of Oncidium.-Biol. Plant. 50: in press, 2006.Google Scholar
  15. Tanaka, M., Hasegawa, A., Goi, M.: Studies on the clonal propagation of monopodial orchids by tissue culture. I. Formation of protocorm-like bodies from leaf tissues in Phalaenopsis and Vanda.-J. jap. Soc. hort. Sci. 44: 47–58, 1975.Google Scholar
  16. Teob, E.S. (ed.): Orchids of Asia.-Times Books International, Singapore 1989.Google Scholar
  17. Tokuhara, K., Masahiro, M.: Micropropagation of Phalaenopsis and Doritaenopsis by culturing shoot tips of flower stalk buds.-Plant Cell Rep. 13: 7–11, 1993.CrossRefGoogle Scholar
  18. Tokuhara, K., Mii, M. Induction of embryogenic callus and cell suspension culture from shoot tips excised from flower stalk buds of Phalaenopsis (Orchidaceae).-In Vitro cell. dev. Biol. Plant 37: 457–461, 2001.Google Scholar
  19. Wang, H.C., Chen, J.T., Chang, W.C.: Somatic embryogenesis and plant regeneration from leaf, root and stem-derived callus cultures of Areca catechu.-Biol. Plant. 50: in press, 2006.Google Scholar
  20. Young, P.S., Murthy, H.N., Yoeup, P.K.: Mass multiplication of protocorm-like bodies using bioreactor system and subsequent plant regeneration in Phalaenopsis.-Plant Cell Tissue Organ Cult. 63: 67–72, 2000.Google Scholar

Copyright information

© Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Praha 2006

Authors and Affiliations

  1. 1.Institute of BiotechnologyNational University of KaohsiungKaohsiungTaiwan, Republic of China
  2. 2.Department of Life SciencesNational University of KaohsiungKaohsiungTaiwan, Republic of China
  3. 3.Institute of Plant and Microbial BiologyAcademia SinicaTaipeiTaiwan, Republic of China

Personalised recommendations