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Biologia Plantarum

, Volume 49, Issue 1, pp 93–97 | Cite as

The effect of NaCl on antioxidant enzyme activities in potato seedlings

  • H. Rahnama
  • H. Ebrahimzadeh
Article

Abstract

The effect of NaCl on the growth and activity of antioxidant enzymes such as superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and ascorbate peroxidase (APX) were investigated in the seedlings of four potato cultivars (Agria, Kennebec; relatively salt tolerant, Diamant and Ajax; relatively salt sensitive). The shoot fresh mass of Agria and Kennebec did not changed at 50 mM NaCl, whereas in Diamant and Ajax it decreased to 50 % of that in the controls. In Agria and Kennebec, SOD activity increased at 50 mM NaCl, but no significant changes observed in Diamant and Ajax. At higher NaCl concentration, SOD activity reduced in all cultivars. CAT and POD activities increased in all cultivars under salt stress. Unlike the other cultivars, in Ajax seedlings, APX activity increased in response to NaCl stress. We also observed new POD and SOD isoenzyme activities and changes in isoenzyme compositions under salt stress. These results suggest that salt-tolerant potato cultivars may have a better protection against reactive oxygen species (ROS) by increasing the activity of antioxidant enzymes (especially SOD) under salt stress.

Additional key words

ascorbate peroxidase catalase peroxidase salt stress Solanum tuberosum superoxide dismutase 

Abbreviations

APX

ascorbate peroxidase (EC 1.11.1.11)

CAT

catalase (EC 1.11.1.6)

NBT

nitroblue tetrazolium

SOD

superoxide dismutase (EC 1.15.1.1)

POD

peroxidase (EC 1.11.1.7)

PVP

polyvinylpyrolidone

GA3

gibberellic acid

NAA

naphthalenacetic acid

STS

silver thiosulfate

EDTA

ethylenediaminetetraacetic acid

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Copyright information

© Institute of Experimental Botany 2005

Authors and Affiliations

  1. 1.Agricultural Biotechnology Research Institute of IranSeed and Plant Improvement Institute CampusKarajIran
  2. 2.Department of Biology, Faculty of ScienceUniversity of TehranTehranIran

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