Biologia Plantarum

, Volume 49, Issue 1, pp 93–97 | Cite as

The effect of NaCl on antioxidant enzyme activities in potato seedlings

  • H. Rahnama
  • H. Ebrahimzadeh


The effect of NaCl on the growth and activity of antioxidant enzymes such as superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and ascorbate peroxidase (APX) were investigated in the seedlings of four potato cultivars (Agria, Kennebec; relatively salt tolerant, Diamant and Ajax; relatively salt sensitive). The shoot fresh mass of Agria and Kennebec did not changed at 50 mM NaCl, whereas in Diamant and Ajax it decreased to 50 % of that in the controls. In Agria and Kennebec, SOD activity increased at 50 mM NaCl, but no significant changes observed in Diamant and Ajax. At higher NaCl concentration, SOD activity reduced in all cultivars. CAT and POD activities increased in all cultivars under salt stress. Unlike the other cultivars, in Ajax seedlings, APX activity increased in response to NaCl stress. We also observed new POD and SOD isoenzyme activities and changes in isoenzyme compositions under salt stress. These results suggest that salt-tolerant potato cultivars may have a better protection against reactive oxygen species (ROS) by increasing the activity of antioxidant enzymes (especially SOD) under salt stress.

Additional key words

ascorbate peroxidase catalase peroxidase salt stress Solanum tuberosum superoxide dismutase 



ascorbate peroxidase (EC


catalase (EC


nitroblue tetrazolium


superoxide dismutase (EC


peroxidase (EC




gibberellic acid


naphthalenacetic acid


silver thiosulfate


ethylenediaminetetraacetic acid


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  1. Abeles, F.B., Biles, C.L.: Characterization of peroxidase in lignifying peach fruit endocarp.-Plant Physiol. 95: 269–273, 1991.PubMedGoogle Scholar
  2. Aebi, H.E.: Catalase.-In: Bergmeyer, H.U. (ed.): Methods of Enzymatic Analysis. Vol. 3. Pp. 273–282. Verlag-Chemie, Weinheim 1983.Google Scholar
  3. Ahmed, R., Abdullah, Z.: Salinity induced changes in the growth and chemical composition of potato.-Pakistan J. Bot. 11: 103–112, 1979.Google Scholar
  4. Arrigoni, O., Garra, L., Tommasi, F., Liso, R.: Changes in ascorbate system during seed development of Vicia faba.-Plant Physiol. 99: 235–238, 1992.PubMedGoogle Scholar
  5. Benavides, M.P., Marconi, P.L., Gallego, S.M., Comba, M.E., Tomaro, M.L.: Relationship between antioxidant defense systems and salt tolerance in Solanum tuberosum.-Aust. J. Plant Physiol. 27: 273–278, 2000.Google Scholar
  6. Bradford, M.M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.-Anal. Biochem. 72: 248–254, 1976.CrossRefPubMedGoogle Scholar
  7. Breusegem, F.V., Vranova, E., Dat, J.F., Inze, D.: The role of active oxygen species in plant signal transduction.-Plant Sci. 161: 405–414, 2001.CrossRefGoogle Scholar
  8. Chang, H., Siegel, B.Z., Sigel, S.M.: Salinity induced changes in isoperoxidase in taro, Colocasia esculenta.-Phytochemistry 23: 233–235, 1984.CrossRefGoogle Scholar
  9. Chen, G., Asada, K.: Ascorbate peroxidase in tea leaves: occurrence of two isozymes and differences in their enzymatic and molecular properties.-Plant Cell Physiol. 30: 987–998, 1989.Google Scholar
  10. Clulow, S.A., Wilkinson, M.J., Burch, L.R.: Solanum phuregia genes are expressed in leaves and tubers of aneusomatic potato dihaploids.-Euphytica 69: 1–6, 1993.CrossRefGoogle Scholar
  11. Davies, K.J.A.: Protein damage and degradation by oxygen radicals. I. General aspects.-J. biol. Chem. 262: 9895–9901, 1987.PubMedGoogle Scholar
  12. Fridovich, I.: Biological effects of superoxide radical.-Arch. Biochem. Biophys. 247: 1–11, 1986.CrossRefPubMedGoogle Scholar
  13. Giannopolitis, C.N., Ries, S.K.: Superoxide dismutase. I. Occurrence in higher plants.-Plant Physiol. 59: 309–314, 1977.CrossRefPubMedGoogle Scholar
  14. Gossett, D.R., Baries, S.W., Millhollon, E.P., Carn Lucas, M.: Antioxidant response to NaCl stress in a control and NaCl tolerant cotton cell line grown in the presence of paraquat, buthionine sulfoximine, and exogenous glutathione.-Plant Physiol. 112: 803–809, 1996.PubMedGoogle Scholar
  15. Gossett, D.R., Millhollon, E.P., Lucas, M.C., Bank, S.W., Marney, M.M.: The effects of NaCl on antioxidant enzyme activities in callus tissue of salt-tolerant and salt-sensitive cotton cultivars (Gossypium hirsutum L.).-Plant Cell Rep. 13: 498–503, 1994.CrossRefGoogle Scholar
  16. Gueta-Dahan, Y., Yaniv, Z., Zilinskas, B.A., Ben-Hayyim, G.: Salt and oxidative stress: similar and specific responses and their relation to salt tolerance in citrus plants.-Planta 203: 460–469, 1997.CrossRefPubMedGoogle Scholar
  17. Halliwell, B., Gutteridge, J.M.C.: Free Radicals in Biology and Medicine.-Clarendon Press, Oxford 1985.Google Scholar
  18. Hames, B.D., Rickwood, D.: Gel Electophoresis of Proteins. A Practical Approach.-Oxford University Press, Oxford 1990.Google Scholar
  19. Hernandez, J.A., Corpas, F.J., Gomez, M., Del Rio, La, Sevilla, F.: Salt induced oxidative stress mediated by activated oxygen species in pea leaf mitochondria.-Physiol. Plant. 89: 103–110, 1993.CrossRefGoogle Scholar
  20. Heuer, B., Nadler, A.: Physiological response of potato plants to soil salinity and water deficit.-Plant Sci. 139: 43–51, 1998.CrossRefGoogle Scholar
  21. Martinez, C.A., Loureiro, M.E., Oliva, M.A., Maesrri, M.: Differential responses of superoxide dismutase in freezing resistant Solanum curtilubum and freezing sensitive Solanum tuberosum subjected to oxidative and water stress.-Plant Sci. 160: 505–515, 2001.CrossRefPubMedGoogle Scholar
  22. Meloni, D.A., Oliva, M.A., Martinez, C.A., Cambraia, J.: Photosynthesis and activity of superoxide dismutase, peroxidase and glutathione reductase in cotton under salt stress.-Environ. exp. Bot. 49: 69–76, 2003.CrossRefGoogle Scholar
  23. Mittler, R., Zilinskas, B.A.: Detection of ascorbate peroxidase activity in native gels by inhibition of the ascorbate-dependent reduction of nitroblue tetrazolium.-Anal. Biochem. 212: 540–546, 1993.CrossRefPubMedGoogle Scholar
  24. Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassay with tobacco tissue culture.-Plant Physiol. 15: 473–497, 1962.CrossRefGoogle Scholar
  25. Núñez, M., Mazzafera, P., Mazorra, L.M., Siquira, W.J., Zullo, M.A.T.: Influence of a brassinosteroid analogue on antioxidant enzymes in rice grown in culture medium with NaCl.-Biol. Plant. 47: 67–70, 2003.CrossRefGoogle Scholar
  26. Rout, N.P., Shaw, B.P.: Salt tolerance in aquatic macrophytes: Possible involvement of the antioxidative enzymes.-Plant Sci. 160: 415–423, 2001.CrossRefPubMedGoogle Scholar
  27. Van Loon, L.C.: Tobacco polyphenol oxidase. A specific staining method indicating non-identify with peroxidase.-Phytochemistry 10: 503–507, 1971.CrossRefGoogle Scholar
  28. Wendel, J.F., Weeden, N.F.: Visualization and interpretation of plant isozymes.-In: Solits, D.E., Solits, P.S. (ed.): Isozymes in Plant Biology. Pp. 5–45. Dioscorides Press, Portland 1990.Google Scholar
  29. Zhu, D., Scandalios, J.G.: Differential accumulation of manganese-superoxide dismutase transcripts in maize in response to abscisic acid and high osmoticum.-Plant Physiol. 106: 177–176, 1994.Google Scholar

Copyright information

© Institute of Experimental Botany 2005

Authors and Affiliations

  1. 1.Agricultural Biotechnology Research Institute of IranSeed and Plant Improvement Institute CampusKarajIran
  2. 2.Department of Biology, Faculty of ScienceUniversity of TehranTehranIran

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