Biologia Plantarum

, Volume 49, Issue 1, pp 23–28 | Cite as

Microspore development during in vitro androgenesis in triticale

  • J. M. GonzálezEmail author
  • N. Jouve


Microspore division was monitored in three triticale (× Triticosecale Wittmack) genotypes over 21 d of in vitro anther culture, on two media differing in their 2,4-dichlorophenoxyacetic acid content. After low temperature (4 °C) pre-treatment for two weeks, all the microspores were still alive, but they began to die from day one of culture. Both genotype and culture medium affected the number of microspores that aborted over time (82 – 97 % by day 21), the number of microspores that underwent the first symmetrical division (> 82 % over all), the number of microspores that attained four or more nuclei, and the number of divisions per 100 alive microspores after 21 d of culture.

Additional key words

anther culture 4-D haploid low temperature × Triticosecale 



2,4-dichlorophenoxyacetic acid


double haploid


embryo-like structures


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Copyright information

© Institute of Experimental Botany 2005

Authors and Affiliations

  1. 1.Departamento de Biología Celular y Genética, Facultad de BiologíaUniversidad de Alcalá, Campus UniversitarioAlcalá de Henares (Madrid)Spain

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