Biologia Plantarum

, Volume 49, Issue 4, pp 541–550 | Cite as

Changes in antioxidant enzymes activity and oxidative stress by abscisic acid and salicylic acid in wheat genotypes

  • S. Agarwal
  • R. K. Sairam
  • G. C. Srivastava
  • R. C. Meena
Article

Abstract

Abscisic acid (ABA) and salicylic acid (SA) were sprayed on leaves of wheat genotypes C 306 and Hira at 25 and 40 d after sowing under moderate water stress (−0.8 MPa) imposed by adding PEG-6000 in nutrient solution. ABA and SA increased the activities of superoxide dismutase, ascorbate peroxidase, glutathione reductase, and catalase in comparison to unsprayed control plants. Both ABA and SA treatments decreased the contents of hydrogen peroxide and thiobarbituric acid reactive substances, a measure of lipid peroxidation, compared to unsprayed plants. The beneficial effect of increase in antioxidant enzymes activity and decrease in oxidative stress was reflected in increase in chlorophyll and carotenoid contents, relative water content, membrane stability index, leaf area and total biomass over control plants. The lower concentrations of ABA (0.5 mM) and SA (1.0 mM) were generally more effective than higher concentrations.

Additional key words

ascorbate peroxidase catalase glutathione reductase hydrogen peroxide lipid peroxidation membrane stability index superoxide dismutase Triticum aestivum 

Abbreviations

ABA

abscisic acid

APOX

ascorbate peroxidase

Car

carotenoids

CAT

catalase

Chl

chlorophyll

DAS

days after sowing

DMSO

dimethylsulfoxide

DTNB

5,5-dithiobis-(2-nitrobenzoic acid)

EDTA

ethylene diamine tetraacetic acid sodium salt

GR

glutathione reductase

GSSG

oxidized glutathione

MSI

membrane stability index

NBT

nitrobluetetrazolium chloride

ROS

reactive oxygen species

SA

salicylic acid

SOD

superoxide dismutase

TBARS

thiobarbituric acid reactive substances

TCA

trichloroacetic acid

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Copyright information

© Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Praha 2005

Authors and Affiliations

  • S. Agarwal
    • 1
  • R. K. Sairam
    • 1
  • G. C. Srivastava
    • 1
  • R. C. Meena
    • 1
  1. 1.Division of Plant PhysiologyIndian Agricultural Research InstituteNew DelhiIndia

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