Biologia Plantarum

, Volume 49, Issue 4, pp 505–511 | Cite as

Seed protein electrophoresis of some cultivated and wild species of Chenopodium

Article

Abstract

Seed protein profiles of 40 cultivated and wild taxa of Chenopodium have been compared by sodium dodecyl sulfate polyacrylamide gel electrophoresis. The relative similarity between various taxa, estimated by Jaccard’s similarity index and clustered in UPGMA dendrogram, is generally in accordance with taxonomic position, crossability relationships and other biochemical characters. Eight accessions of C. quinoa studied are clustered together and show genetic similarity with closely related C. bushianum and C. berlandieri subsp. nuttalliae. The taxa included under C. album complex are clustered in two groups which show that these taxa are a heterogenous assemblage and their taxonomic affinities need a reassessment. Other wild species studied are placed in the dendrogram more or less according to their taxonomic position.

Additional key words

genetic diversity Jaccard’s similarity index SDS-PAGE taxonomy UPGMA dendrogram 

Abbreviations

NTSYS-PC

numerical taxonomy and multivariate analysis system

OTU

operational taxonomic unit

PAGE

polyacrylamide gel electrophoresis

SDS

sodiumdodecyl sulphate

UPGMA

unweighted pair-group method

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Ahmad, F., Slinkard, A.E.: Genetic relationships in the genus Cicer L. as revealed by polyacrylamide gel electrophoresis of seed storage proteins.-Theor. appl. Genet. 84: 688–692, 1992.CrossRefGoogle Scholar
  2. Bera, B., Mukherjee, K.K.: Phenotypic variability in Chenopodium album.-Nucleus 30: 50–53, 1987.Google Scholar
  3. El Naggar, S.M.: Implications of seed proteins in Brassicaceae systematics.-Biol. Plant. 44: 547–553, 2001.CrossRefGoogle Scholar
  4. Gangopadhyay, G., Das, S., Mukherjee, K.K.: Speciation in Chenopodium in West Bengal, India.-Genet. Res. Crop Evol. 49: 503–510, 2002.CrossRefGoogle Scholar
  5. Ghafoor, A., Ahmad, Z., Qureshi, A.S., Bashir, M.: Genetic relationship in Vigna mungo (L.) Hepper and V. radiata (L.) R. Wilczek based on morphological traits and SDS-PAGE.-Euphytica 123: 367–378, 2002.CrossRefGoogle Scholar
  6. Giusti, L.: [El genero Chenopodium in Argentina I. Numero de cromosomas.]-Darwiniana 16: 98–105, 1970. [In Span.]Google Scholar
  7. Heiser, C.B., Nelson, D.C.: On the origin of cultivated chenopods (Chenopodium).-Genetics 78: 503–505, 1974.PubMedGoogle Scholar
  8. Jha, S.S., Ohri, D.: Phylogenetic relationships of Cajanus cajan (L.) Millsp. (pigeon pea) and its wild relatives based on seed protein profiles.-Genet. Res. Crop Evol. 43: 275–281, 1996.CrossRefGoogle Scholar
  9. Laemmli, U.K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4-Nature 227: 680–685, 1970.PubMedGoogle Scholar
  10. Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randal, R.J.: Protein measurement with the Folin phenol reagent.-J. biol. Chem. 193: 265–275, 1951.PubMedGoogle Scholar
  11. Mehra, P.N., Malik, C.P.: Cytology of some Indian Chenopodiaceae.-Caryologia 16: 67–84, 1963.Google Scholar
  12. Mosyakin, S.L., Clemants, S.E.: New infrageneric taxa and combinations in Chenopodium L. (Chenopodiaceae).-Novon 6: 398–403, 1996.Google Scholar
  13. Mukherjee, K.K.: A comparative study of two cytotpes of Chenopodium album in West Bengal, India.-Can. J. Bot. 64: 754–759, 1986.Google Scholar
  14. Nath, P., Ohri, D., Jha, S.S., Pal, M.: Seed protein electrophoresis of wild and cultivated species of Celosia (Amaranthaceae).-Genet. Res. Crop Evol. 44: 241–245, 1997.CrossRefGoogle Scholar
  15. Pal, M., Shukla, S.: A hexaploid grain chenopod from Eastern Himalayas.-Newslett. Himalayan Bot. 8: 12–14, 1990.Google Scholar
  16. Partap, T., Joshi, B.D., Galwey, N.W.: Chenopods: Chenopodium spp. Promoting the Conservation and Use of Underutilized and Neglected Crops.-Institute of Plant Genetics and Crop Plant Research, Gatersleben/International Plant Genetic Resources Institute, Rome 1998.Google Scholar
  17. Risi, J., Galwey, N.W.: The Chenopodium grains of the Andes: Inca crops for modern agriculture.-Adv. appl. Biol. 10: 146–206, 1984.Google Scholar
  18. Ruas, P.M, Bonifacio, A., Ruas, C.F., Fairbanks, D.J., Anderson, W.R.: Genetic relationships among 19 accessions of six species of Chenopodium L. by random amplified polymorphic DNA fragments (RAPD).-Euphytica 105: 25–32, 1999.CrossRefGoogle Scholar
  19. Scott, A.J.: A review of the classification of Chenopodium L. and related genera (Chenopodiaceae).-Bot. Jahrb. Syst. 100: 205–220, 1978.Google Scholar
  20. Sneath, P.H.A., Sokal, R.R.: Numerical Taxonomy.-W.H. Freeman, San Francisco 1973.Google Scholar
  21. Vladova, R., Pandeva, R., Petcolicheva, K.: Seed storage proteins in Capsicum annum cultivars.-Biol. Plant. 43: 291–295, 2000.CrossRefGoogle Scholar
  22. Walters, T.W.: Electrophoretic evidence for the evolutionary relationship of the tetraploid Chenopodium berlandieri to its putative diploid progenitors.-Selbyana 10: 36–55, 1987.Google Scholar
  23. Wilson, H.D.: Genetic control and distribution of leucine aminopeptidase in the cultivated chenopods (Chenopodiaceae) and related weed taxa.-Biochem. Genet. 14: 913–919, 1976.CrossRefPubMedGoogle Scholar
  24. Wilson, H.D.: Artificial hybridization among species of Chenopodium sect. Chenopodium.-Syst. Bot. 5: 253–263, 1980.Google Scholar
  25. Wilson, H.D.: Genetic variations among South American populations of tetraploid Chenopodium sect. Chenopodium subsect. Cellulata.-Syst. Bot. 6: 380–398, 1981.Google Scholar
  26. Wilson, H.D.: Allozyme variation and morphological relationships of Chenopodium hircinum Schrader (s.lat.).-Syst. Bot. 13: 215–228, 1988a.Google Scholar
  27. Wilson, H.D.: Quinua biosystematics I. Domesticated populations.-Econ. Bot. 42: 461–477, 1988b.Google Scholar
  28. Wilson, H.D.: Quinua biosystematics II. Free living populations.-Econ. Bot. 42: 478–494, 1988c.Google Scholar
  29. Wilson, H.D.: Quinoa and relatives (Chenopodium sect. Chenopodium subsect. Cellulata).-Econ. Bot. 44: 92–110, 1990.Google Scholar
  30. Wilson, H.D., Heiser, C.B.: The origin and evolutionary relationships of ‘Huazontle’ (Chenopodium nuttalliae Safford) domesticated chenopod of Mexico.-Amer. J. Bot. 66: 198–206, 1979.Google Scholar

Copyright information

© Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Praha 2005

Authors and Affiliations

  1. 1.National Botanical Research InstituteLucknowIndia

Personalised recommendations