Biologia Plantarum

, Volume 49, Issue 3, pp 427–430 | Cite as

Thidiazuron induced adventitious shoot regeneration in Hyoscyamus niger

  • S. Uranbey
Brief Communication


A high frequency adventitious shoot regeneration protocol was developed for henbane (Hyoscyamus niger L.) using thidiazuron (TDZ). Hypocotyl, cotyledon and stem explants were cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of N6-benzylaminopurine and TDZ. MS medium supplemented with 16 μM TDZ was the most effective for providing 100 % regeneration frequency associated with a 19.53 shoots per hypocotyl explant. Plantlets were rooted on MS medium supplemented with different concentrations of indole-3-butyric acid (IBA) and α-naphthaleneacetic acid. High rooting and survival was achieved using half strength MS medium supplemented with 8 μM IBA.

Additional key words

N6-benzylaminopurine henbane indole-3-butyric acid α-naphthaleneacetic acid rooting tissue culture 





indole-3-acetic acid


indole-3-butyric acid


Murashige and Skoog medium


α-naphthaleneacetic acid




Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Cheng, J., Raghavan, V.: Somatic embryogenesis and plant regeneration Hyoscyamus niger.-Amer. J.Bot. 72: 580–587, 1985.Google Scholar
  2. Hosseini-Nasr, M., Rashid, A.: Thiadiazuron-induced high frequency shoot regeneration from root region of Robinia pseudoacacia L. seedlings.-Biol. Plant. 47: 591–596, 2003/4.Google Scholar
  3. Kanyad, M., Dessai, A.P., Prakash, C.S.: Thidiazuron promotes high frequency regeneration of peanut (Arachis hypogaea) plants in vitro.-Plant Cell Rep. 14: 1–5, 1994.Google Scholar
  4. Kim, M.K., Sommer, H.E., Bongarten, B.C., Merkle, S.A. High frequency induction of adventitious shoots from hypocotyl segments of Liquidambar styraciflua L. by thidiazuron.-Plant Cell Rep. 16: 536–540, 1997.Google Scholar
  5. Koroch, A., Juliani, H.R., Kapteyn, J., Simon, J.E.: In vitro regeneration of Echinacea purpurea from leaf explants.-Plant Cell Tissue Organ Cult. 69: 79–83, 2002.CrossRefGoogle Scholar
  6. Leo, A., Zeef., Christou, P., Leech, M.J.: Transformation of the tropane alkoloid-producing medicinal plant Hyoscyamus muticus by particle bombardment.-Transgenic Res. 9: 163–168, 2000.CrossRefPubMedGoogle Scholar
  7. Lu, C.Y.: The use of thidiazuron in tissue culture.-In vitro cell. dev. Biol. Plant 29: 92–96, 1993.Google Scholar
  8. Malik, K.A. Saxena, P.K.: Regeneration in Phaseolus vulgaris L.: High frequency induction of direct shoot formation in intact seedlings by N6-benzylaminopurine and thidiazuron.-Planta 186: 384–389, 1992.CrossRefGoogle Scholar
  9. Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures.-Physiol. Plant. 15: 473–497, 1962.Google Scholar
  10. Raghavan, V., Nagmani, R.: Cytokinin effects on pollen embryogenesis in cultured anthers of Hyoscyamus niger.-Can. J. Bot. 67: 247–257, 1989.Google Scholar
  11. Sevon, N., Oksman-Caldentey, K.M., Hiltunen, R.: Efficent plant regeneration from hairy root-derived protoplast of Hyoscyamus muticus.-Plant Cell Rep. 14: 738–742, 1995.Google Scholar
  12. Snedecor, G.W., Cochran, W.G.: Statistical Methods.-The Iowa State University Press, Iowa 1967.Google Scholar
  13. Thomas, T.D.: Thiadiazuron induced multiple shoot induction and plant regeneration from cotyledonary explants of mulberry.-Biol. Plant. 46: 529–533, 2003.CrossRefGoogle Scholar
  14. Uranbey, S., Cocu, S., Sancak, C., Parmaksız, I., Khawar, K.M., Mirici, S., Ozcan, S.: Efficient aventitious shoot regeneration system in cicer milkvetch.-Biotech. Biotech. Equip. 17: 33–37, 2003.Google Scholar
  15. Uranbey, S., Sevinay, C.S., Ozcan, S.: Development of high frequency multiple shoot formation in Persian clover (Trifolium resupinatum L.).-Plant Cell Tissue Organ Cult. 80: 229–232, 2005.CrossRefGoogle Scholar
  16. Wilhelm, E.: Micropropagation of juvenile sycamore maple via adventitious shoot formation by use of thidiazuron.-Plant Cell Tissue Organ Cult. 57: 57–60, 1999.CrossRefGoogle Scholar

Copyright information

© Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Praha 2005

Authors and Affiliations

  1. 1.Ministry of Agriculture and Rural AffairsCentral Research Institute for Field CropsAnkaraTurkey

Personalised recommendations