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Biologia Plantarum

, Volume 49, Issue 3, pp 427–430 | Cite as

Thidiazuron induced adventitious shoot regeneration in Hyoscyamus niger

  • S. Uranbey
Brief Communication

Abstract

A high frequency adventitious shoot regeneration protocol was developed for henbane (Hyoscyamus niger L.) using thidiazuron (TDZ). Hypocotyl, cotyledon and stem explants were cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of N6-benzylaminopurine and TDZ. MS medium supplemented with 16 μM TDZ was the most effective for providing 100 % regeneration frequency associated with a 19.53 shoots per hypocotyl explant. Plantlets were rooted on MS medium supplemented with different concentrations of indole-3-butyric acid (IBA) and α-naphthaleneacetic acid. High rooting and survival was achieved using half strength MS medium supplemented with 8 μM IBA.

Additional key words

N6-benzylaminopurine henbane indole-3-butyric acid α-naphthaleneacetic acid rooting tissue culture 

Abbreviations

BAP

N6-benzylaminopurine

IAA

indole-3-acetic acid

IBA

indole-3-butyric acid

MS

Murashige and Skoog medium

NAA

α-naphthaleneacetic acid

TDZ

thidiazuron

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Copyright information

© Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Praha 2005

Authors and Affiliations

  1. 1.Ministry of Agriculture and Rural AffairsCentral Research Institute for Field CropsAnkaraTurkey

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