The bioaccumulation of waterborne zinc in tissues of silver catfish (Rhamdia quelen) and its effect on biochemical parameters
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Silver catfish (Rhamdia quelen) is a fish species with neotropical distribution, and is a potential model organism to study polluted environment. The aim of this study is to analyze the response of silver catfish to environmental concentrations of waterborne zinc (Zn) over 96 h. Significant metal accumulation was seen in gill, intestine and liver tissues. No significant accumulation was seen in muscle tissue. Lipid peroxidation increased in the brain, and decreased in the muscle and liver at all levels of exposure. Zinc exposure led to decreased protein carbonyl levels in the brain and increased levels in the liver. The activity of catalase in the liver was reduced for all exposed groups. Glutathione S-transferase activity decreased in the brain at the highest level of exposure and in the liver at all Zn concentrations tested. Non-protein thiols increased in the muscle and in the gills after exposure. Ascorbic acid levels increased in the brain and in the gills. Exposure to Zn also altered the metabolic parameters, causing decreased lactate and ammonia levels in the muscle, and decreased glycogen in the liver. Zinc exposure increased ammonia and amino acid levels in the liver, and increase glycogen and amino acid levels in muscle tissue. Our results demonstrate that exposure to environmentally relevant concentrations of Zn led to accumulation of metals in the tissues of silver catfish, with significant changes in biochemical parameters.
KeywordsAquatic contamination Environmental concentrations Fish Metal
This study was supported in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES) – Programa PROEX Protocol Number: 23038.005848/2018-31. Student Jossiele Leitemperger received fellowship from CAPES under Protocol Number 88882.182137/2018-01 and student Tiago Fiuza received fellowship from CAPES under Protocol Number 88882.182157/2018-01. Vania Lucia Loro received research fellowship from CNPq under Process Number: 309314/2017-8.
- Bidinotto PM, Moraes G, Souza RHS (1997) Hepatic glycogen and glucose in eight tropical fresh water teleost fish: aprocedure for field determinations of micro samples. Bol Tec CEPTA Pirassununga 10:53–60Google Scholar
- CONAMA (Conselho Nacional do Meio Ambiente) Resolução CONAMA nº 357 de 17/03/05. http://www.mma.gov.br/port/conama/legiabre.cfm?codleg=459. Accessed 20 June 2018
- Eisler R (1988) Lead hazards to fish, wildlife, and invertebrates: a synoptic review. US Fish and Wildlife Service. Biological Report 85(1.14)Google Scholar
- FAO/WHO (1983) Compilation of legal limits for hazardous substances in fish and fishery products. Fish Circ 464:764Google Scholar
- FAO/WHO (1989) National research council recommended dietary allowances, 10th edn. National Academy Press, Washington, DCGoogle Scholar
- Habig WH, Pabst MJ, Jacoby WB (1974) Glutathione S-transferase, the first enzymatic step in mercapturic acid formation. J Biol Chem 249:7130–7139Google Scholar
- Lowry DH, Rosenbrough NJ, Far AL, Randal RJ (1951) Protein measurement with Folin phenol reagent. J Biol Chem 193:265–275Google Scholar
- Misra HP, Fridovich I (1972) The role of superoxide anion in the auto-oxidation of epinephrine and a simple assay for superoxide dismutase. J Biol Chem 247:3170–3175Google Scholar
- Pretto A, Loro VL, Silva VM, Salbego J, Menezes CC, Souza CF, Gioda CR, Baldisserotto B (2014b) Exposure to sublethal concentrations of copper changes biochemistry parameters in silver catfish, Rhamdia quelen, Quoy & Gaimard. Bull Environ Contam Toxicol 92(4):399–403. https://doi.org/10.1007/s00128-014-1215-8 CrossRefGoogle Scholar
- Roe JH (1954) In: Glick D (ed) Methods of biochemical analysis. Interscience Publishers, New York, pp 115–139Google Scholar
- Romani R, Antognelli C, Baldracchini F, De Santis A, Isani G, Giovannini E, Rossi G (2003) Increased acetylcholinesterase activities in specimens of Sparus auratus exposed to sublethal copper concentrations. Chem Biol Interact 145:321–329. https://doi.org/10.1016/S0009-2797(03)00058-9 CrossRefGoogle Scholar