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BioMetals

, Volume 32, Issue 1, pp 77–88 | Cite as

Algae induce siderophore biosynthesis in the freshwater bacterium Cupriavidus necator H16

  • Colette Kurth
  • Ina Wasmuth
  • Thomas Wichard
  • Georg Pohnert
  • Markus NettEmail author
Article
  • 239 Downloads

Abstract

Cupriachelin is a photoreactive lipopeptide siderophore produced by the freshwater bacterium Cupriavidus necator H16. In the presence of sunlight, the iron-loaded siderophore undergoes photolytic cleavage, thereby releasing solubilized iron into the environment. This iron is not only available to the siderophore producer, but also to the surrounding microbial community. In this study, the cupriachelin-based interaction between C. necator H16 and the freshwater diatom Navicula pelliculosa was investigated. A reporter strain of the bacterium was constructed to study differential expression levels of the cupriachelin biosynthesis gene cucJ in response to varying environmental conditions. Not only iron starvation, but also culture supernatants of N. pelliculosa were found to induce cupriachelin biosynthesis. The transcription factors involved in this differential gene expression were identified using DNA–protein pulldown assays. Besides the well-characterized ferric uptake regulator, a two-component system was found to tune the expression of cupriachelin biosynthesis genes in the presence of diatom supernatants.

Keywords

Siderophore Cupriavidus necator Diatom Interaction Freshwater 

Notes

Acknowledgements

This project was supported by the Collaborative Research Center ChemBioSys (CRC1127 ChemBioSys) and funded by the Deutsche Forschungsgemeinschaft. We thank T. Kindel (Hans Knöll Institute Jena, Department for Molecular and Applied Microbiology) for MALDI-TOF/TOF measurements.

Supplementary material

10534_2018_159_MOESM1_ESM.pdf (469 kb)
Supplementary material 1 (PDF 469 kb)

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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  1. 1.Leibniz Institute for Natural Product Research and Infection BiologyHans Knöll InstituteJenaGermany
  2. 2.Institute for Inorganic and Analytical ChemistryFriedrich Schiller University JenaJenaGermany
  3. 3.Laboratory of Technical Biology, Department of Biochemical and Chemical EngineeringTU Dortmund UniversityDortmundGermany

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