Citrate analysis using capillary electrophoresis and complexation with Eu3+-tetracycline
A sensitive assay for citrate was developed. Citrate was incubated with 50 μM Eu3+-tetracycline and the complex separated using capillary electrophoresis utilizing post-column laser-induced luminescence detection in a sheath flow cuvette. Signal was linear with citrate concentration from 10 μM to 200 nM. Injection volumes were 320 pL. For the 200 nM sample, this corresponds to the injection of 64 amoles of citrate. Separation time was < 90 s with a total run time of 5 min. As an application the method was used to analyze citrate in agricultural and medicinal products. The method was also used to develop an assay for the enzyme citrate synthase.
KeywordsCapillary electrophoresis Citrate Citrate synthase Eu3+-tetracycline Luminescence
This study was supported by a Grant from the Natural Sciences and Engineering Research Council.
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