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BioMetals

, Volume 31, Issue 1, pp 139–146 | Cite as

Evaluation of the iron regulatory protein-1 interactome

  • Gregory J. Connell
  • Jando S. Danial
  • Christian X. Haastruthers
Article

Abstract

The interactions of iron regulatory proteins (IRPs) with mRNAs containing an iron-responsive element (IRE) is a major means through which intracellular iron homeostasis is maintained and integrated with cellular function. Although IRE–IRP interactions have been proposed to modulate the expression of a diverse number of mRNAs, a transcriptome analysis of the interactions that form within the native mRNA structure and cellular environment has not previously been described. An RNA-CLIP study is described here that identified IRP-1 interactions occurring within a primary cell line expressing physiologically relevant amounts of mRNA and protein. The study suggests that only a small subset of the previously proposed IREs interact with IRP-1 in situ. Identifying authentic IRP interactions is not only important to a greater understanding of iron homeostasis and its integration with cell biology but also to the development of novel therapeutics that can compensate for iron imbalances.

Keywords

Iron homeostasis Iron-responsive elements Iron regulatory protein Interactome RNA-CLIP 

Notes

Acknowledgements

This work was funded in part by a grant from the University of Minnesota Academic Health Center to GJC (Grant Number 12.04). We also acknowledge the assistance of the University of Minnesota Genomics Center and the University of Minnesota Supercomputing Institute.

Funding

This work was supported in part by a grant from the University of Minnesota Academic Health Center to GJC (Grant Number 12.04).

Compliance with ethical standards

Conflict of interests

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of PharmacologyUniversity of Minnesota Medical SchoolMinneapolisUSA

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