Proteome wide identification of iron binding proteins of Xanthomonas translucens pv. undulosa: focus on secretory virulent proteins
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Xanthomonas translucens pv. undulosa (Xtu) causes Bacterial Leaf Streak disease in the staple food crops such as wheat and barley. The survival strategies of pathogen and host are determined by the complex interactions occurring between the host plants and the pathogenic microbes. Iron binding proteins are important in the plant–microbe interactions as they are indulged in enzyme catalysis, virulence, metabolic and transport activities. In the presented study, we have identified that ~9.8% of Xtu proteome possess iron binding sequence motifs. Further, the analysis of Xtu proteome for secretory iron binding virulent proteins (IBVPs) revealed the fact that iron co-regulate the function of secretory proteins in virulence. We have found 26 secretory IBVPs and observed that these proteins are diverse in their biological functions ranging from transport to antimicrobial resistance, Reactive oxygen species detoxification and carbohydrate catabolism. The inferences may instigate to design the new strategies to combat and control the microbial diseases of staple food crops.
KeywordsXanthomonas translucens pv. undulosa Iron binding proteins Secretory iron binding virulent proteins Ton-B dependent receptors
Shailender Kumar Verma’s lab. is funded by Department of Biotechnology, Ministry of Science and Technology, Government of India (Project No. BT/PR13692/BID/7/543/2015). University Grant Commission, India is acknowledged for providing stipends to Ms. Ankita Sharma and Mr. Dixit Sharma. Authors are thankful to Central University of Himachal Pradesh (CUHP) for providing computational facilities. Authors are also grateful to all the members of Biosciences Study Circle, CUHP for their valuable suggestions.
Compliance with ethical standards
Conflict of interest
Authors declare no conflict of interest.
- Bull CT, Deboer SH, Denny TP, Firrao G, Saux MF, Saddler GS, Scortichini M, Stead DE, Takikawa Y (2010) Comprehensive list of names of plant pathogenic bacteria 1980-2007. J Plant Pathol 92:551–592Google Scholar
- DeLano WL (2002) The PyMOL molecular graphics system. DeLano Scientific, Palo AltoGoogle Scholar
- Duveiller E, Maraite H (1993) Study on yield loss due to Xanthomonas campestris pv. undulosa in wheat under high rainfall temperate conditions. J Plant Dis Prot 100:453–459Google Scholar
- Gennaris A, Ezraty B, Henry C, Agrebi R, Vergnes A, Oheix E, Bos J, Leverrier P, Espinosa L, Szewczyk J, Vertommen D, Iranzo O, Collet JF, Barras F (2015) Repairing oxidized proteins in the bacterial envelope using respiratory chain electrons. Nature 528:409–412CrossRefPubMedPubMedCentralGoogle Scholar
- Hassett DJ, Ma JF, Elkins JG, McDermott TR, Ochsner UA, West SE, Huang CT, Fredericks J, Burnett S, Stewart PS, McFeters G, Passador L, Iglewski BH (1999) Quorum sensing in Pseudomonas aeruginosa controls expression of catalase and superoxide dismutase genes and mediates biofilm susceptibility to hydrogen peroxide. Mol Microbiol 34:1082–1093CrossRefPubMedGoogle Scholar
- Jensen LJ, Gupta R, Blom N, Devos D, Tamames J, Kesmir C, Nielsen H, Staerfeldt HH, Rapacki K, Workman C, Andersen CA, Knudsen S, Krogh A, Valencia A, Brunak S (2002) Prediction of human protein function from post-translational modifications and localization features. J Mol Biol 319:1257–1265CrossRefPubMedGoogle Scholar
- Mathee K, Ciofu O, Sternberg C, Lindum PW, Campbell JI, Jensen P, Johnsen AH, Givskov M, Ohman DE, Molin S, Høiby N, Kharazmi A (1999) Mucoid conversion of Pseudomonas aeruginos by hydrogen peroxide: a mechanism for virulence activation in the cystic fibrosis lung. Microbiology 145:1349–1357CrossRefPubMedGoogle Scholar
- Prasannath K (2013) Pathogenicity and virulence factors of phytobacteria. Sch Acad J Biosci 1:24–33Google Scholar
- Rott P, Fleites L, Marlow GC, Royer M, Gabriel DW (2009) An OmpA family outer membrane protein is required for both disease symptom development and sugarcane stalk colonization by Xanthomonas albilineans. Phytopathology 99:S110–S111Google Scholar
- Rubartellisg A, Bajettos A, Allavenaz G, Wollman E, Sitia R (1992) Secretion of thioredoxin by normal and neoplastic cells through a leaderless secretory. J Biol Chem 267:24161–24164Google Scholar
- Marchler-Bauer A, Derbyshire MK, Gonzales NR, Lu S, Chitsaz F, Geer LY, Geer RC, He J, Gwadz M, Hurwitz DI, Lanczycki CJ, Lu F, Marchler GH, Song JS, Thanki N, Wang Z, Yamashita RA, Zhang D, Zheng C, Bryant SH (2015) CDD: NCBI’s conserved domain database. Nucleic Acids Res 43:D222–226CrossRefPubMedGoogle Scholar
- Yu NY, Wagner JR, Laird MR, Melli G, Rey S, Lo R, Dao P, Sahinalp SC, Ester M, Foster LJ, Brinkman FS (2010) PSORTb 3.0: improved protein subcellular localization prediction with refined localization subcategories and predictive capabilities for all prokaryotes. Bioinformatics 26:1608–1615CrossRefPubMedPubMedCentralGoogle Scholar