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BioMetals

, Volume 29, Issue 1, pp 95–106 | Cite as

Contribution of arginase to manganese metabolism of Aspergillus niger

  • Sarita Keni
  • Narayan S. PunekarEmail author
Article

Abstract

Aspects of manganese metabolism during normal and acidogenic growth of Aspergillus niger were explored. Arginase from this fungus was a Mn[II]-enzyme. The contribution of the arginase protein towards A. niger manganese metabolism was investigated using arginase knockout (D-42) and arginase over-expressing (ΔXCA-29) strains of A. niger NCIM 565. The Mn[II] contents of various mycelial fractions were found in the order: D-42 strain < parent strain < ΔXCA-29 strain. While the soluble fraction forms 60 % of the total mycelial Mn[II] content, arginase accounted for a significant fraction of this soluble Mn[II] pool. Changes in the arginase levels affected the absolute mycelial Mn[II] content but not its distribution in the various mycelial fractions. The A. niger mycelia harvested from acidogenic growth media contain substantially less Mn[II] as compared to those from normal growth media. Nevertheless, acidogenic mycelia harbor considerable Mn[II] levels and a functional arginase. Altered levels of mycelial arginase protein did not significantly influence citric acid production. The relevance of arginase to cellular Mn[II] pool and homeostasis was evaluated and the results suggest that arginase regulation could occur via manganese availability.

Keywords

Aspergillus niger Arginase Manganese metabolism Acidogenic growth 

Notes

Acknowledgments

We acknowledge Sophisticated Analysis and Instrumentation Facility (SAIF), Department of Earth Sciences, and Centre for Environmental Science and Engineering (CESE) at IIT Bombay for support in Mn[II] analyses. Dr. Neetu Singh performed early standardizations of A. niger growth inhibition by Mn[II]. The work was supported by a grant from Board of Research in Nuclear Sciences (BRNS), Department of Atomic Energy (DAE) and fellowship (to Sarita Keni) by Council of Scientific and Industrial Research (CSIR).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Department of Biosciences and BioengineeringIndian Institute of Technology BombayMumbaiIndia

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