BioMetals

, Volume 29, Issue 1, pp 53–60 | Cite as

Characterization of the iron-binding properties of pyoverdine using electron-capture dissociation-tandem mass spectrometry

Article
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Abstract

Pyoverdines (PVD) are a group of siderophores produced by fluorescent Pseudomonads. Identification of PVD variants mostly relies on liquid chromatography-tandem mass spectrometry (LC–MS/MS) using collision-induced dissociation (CID). Here, both CID and the novel dissociation technique electron-capture dissociation (ECD) were applied to characterize PVD succinamide and its Fe(III)-chelated complex. The results clearly showed that ECD produced diagnostic side chain fragmentation of the PVD peptide chain and preserved the labile Fe(III) binding to the chromophore in contrast to CID. The ECD technique is therefore expected to support the understanding of strain-specific Fe(III) transport processes of PVDs.

Keywords

Pyoverdine Siderophores Iron binding FTICR-MS Electron-capture dissociation ECD 
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Notes

Acknowledgments

DAV acknowledges general research support by the Alfried Krupp von Bohlen und Halbach-Stiftung. This work was supported in part by the DFG FTICR-MS Facility, INST 256/356-1.

Supplementary material

10534_2015_9895_MOESM1_ESM.docx (153 kb)
Supplementary material 1 (DOCX 153 kb)

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Institute of Bioanalytical ChemistrySaarland UniversitySaarbrückenGermany
  2. 2.Institute of Inorganic and Analytical ChemistryUniversity of MünsterMünsterGermany

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