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BioMetals

, Volume 27, Issue 6, pp 1337–1349 | Cite as

Investigation of metal sorption behavior of Slp1 from Lysinibacillus sphaericus JG-B53: a combined study using QCM-D, ICP-MS and AFM

  • Matthias SuhrEmail author
  • Nancy Unger
  • Karen E. Viacava
  • Tobias J. Günther
  • Johannes Raff
  • Katrin Pollmann
Article

Abstract

Surface layer proteins (S-layer) of Lysinibacillus sphaericus JG-B53 are biological compounds with several bio-based technical applications such as biosorptive materials for metal removal or rare metals recovery from the environment. Despite their well-described applications, a deeper understanding of their metal sorption behavior still remains challenging. The metal sorption ability of Au3+, Pd2+, Pt2+ and Eu3+ was investigated by ICP-MS, AFM and QCM-D which enables the sorption detection in real-time during in situ experiments. Results indicate a high binding of Pd, followed by Au, Eu and Pt to the proteins. The comparison between different methods allowed a deeper understanding of the metal sorption of isolated S-layer either frees in liquid, adsorbed forming a protein layer or as the bacteria surface.

Keywords

Biosorption Metals S-layer QCM-D AFM ICP-MS 

Notes

Acknowledgments

The present work was partially funded by the BMBF-project BIONA (BMBF/DLR 01RB0805A). The author of this paper would like to thank Aline Ritter for her valuable support with ICP-MS measurements. Special thanks to Prof. E. Brunner (TU Dresden), Prof. J.-U. Ackermann (HTW Dresden).

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Matthias Suhr
    • 1
    Email author
  • Nancy Unger
    • 1
  • Karen E. Viacava
    • 1
  • Tobias J. Günther
    • 2
  • Johannes Raff
    • 1
    • 2
  • Katrin Pollmann
    • 2
  1. 1.Institute of Resource EcologyHelmholtz-Zentrum Dresden-RossendorfDresdenGermany
  2. 2.Helmholtz-Institute Freiberg for Resource TechnologyHelmholtz-Zentrum Dresden-RossendorfDresdenGermany

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