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BioMetals

, Volume 27, Issue 4, pp 633–644 | Cite as

Analysis of the draft genome of Pseudomonas fluorescens ATCC17400 indicates a capacity to take up iron from a wide range of sources, including different exogenous pyoverdines

  • Lumeng Ye
  • Sandra Matthijs
  • Josselin Bodilis
  • Falk Hildebrand
  • Jeroen Raes
  • Pierre CornelisEmail author
Article

Abstract

All fluorescent pseudomonads (Pseudomonas aeruginosa, P. putida, P. fluorescens, P. syringae and others) are known to produce the high-affinity peptidic yellow-green fluorescent siderophore pyoverdine. These siderophores have peptide chains that are quite diverse and more than 50 pyoverdine structures have been elucidated. In the majority of the cases, a Pseudomonas species is also able to produce a second siderophore of lower affinity for iron. Pseudomonas fluorescens ATCC 17400 has been shown to produce a unique second siderophore, (thio)quinolobactin, which has an antimicrobial activity against the phytopathogenic Oomycete Pythium debaryanum. We show that this strain has the capacity to utilize 16 different pyoverdines, suggesting the presence of several ferripyoverdine receptors. Analysis of the draft genome of P. fluorescens ATCC 17400 confirmed the presence of 55 TonB-dependent receptors, the largest so far for Pseudomonas, among which 15 are predicted to be ferripyoverdine receptors (Fpv). Phylogenetic analysis revealed the presence of two different clades containing ferripyoverdine receptors, with sequences similar to the P. aeruginosa type II FpvA forming a separate cluster. Among the other receptors we confirmed the presence of the QbsI (thio)quinolobactin receptor, an ferri-achromobactin and an ornicorrugatin receptor, several catecholate and four putative heme receptors. Twenty five of the receptors genes were found to be associated with genes encoding extracytoplasmic sigma factors (ECF σ) and transmembrane anti-σ sensors.

Keywords

Pseudomonas fluorescens Siderophores Pyoverdines Quinolobactin Heme TonB-dependent receptors 

Supplementary material

10534_2014_9734_MOESM1_ESM.doc (170 kb)
Supplementary material 1 (DOC 169 kb)

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Lumeng Ye
    • 1
  • Sandra Matthijs
    • 2
  • Josselin Bodilis
    • 3
  • Falk Hildebrand
    • 1
  • Jeroen Raes
    • 1
  • Pierre Cornelis
    • 1
    Email author
  1. 1.Department of Bioengineering Sciences, Research Group Microbiology, VIB Structural BiologyVrije Universiteit BrusselBrusselsBelgium
  2. 2.Institut de Recherches Microbiologiques-WiameBrusselsBelgium
  3. 3.Laboratoire de Microbiologie Signaux et MicroenvironnementUFR des Sciences - Université de RouenMont Saint AignanFrance

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