Antifungal activity of ZnO nanoparticles and their interactive effect with a biocontrol bacterium on growth antagonism of the plant pathogen Fusarium graminearum
- 1.4k Downloads
Fungal plant pathogens such as Fusarium graminearum cause severe global economic losses in cereals crops, and current control measures are limited. This work addresses the potential for ZnO nanoparticles (NPs) and biocontrol bacteria to be used in plant fungal control strategies. Growth of F. graminearum was significantly (p = 0.05) inhibited by inclusion of the NPs in a mung bean broth agar and in sand. Suspension in mung bean broth medium modified the surface charge, dissolution, and aggregation state of the ZnO NPs, in comparison to processes occurring in water suspension. The ZnO NPs were significantly more inhibitory to fungal growth than micro-sized particles of ZnO, although both types of particles released similar levels of soluble Zn, indicating size-dependent toxicity of the particles. Zn ions produced dose-dependent inhibition, noticeable at the level of soluble Zn released from NPs after seven-day suspension in medium; inhibitory levels caused acidification of the growth medium. Transfer of fungal inoculum after exposure to the ZnO NPs to fresh medium did not indicate adaptation to the stress because growth was still inhibited by the NPs. The ZnO NPs did not prevent metabolites from a biocontrol bacterium, Pseudomonas chlororaphis O6, from inhibiting Fusarium growth: no synergism was observed in the mung bean agar. Because other studies find that soil amendment with ZnO NPs required high doses for inhibition of plant growth, the findings of pathogen growth control reported in this paper open the possibility of using ZnO NP-based formulations to complement existing strategies for improving crop health in field settings.
KeywordsBiocontrol Fungi Fusarium graminearum Nanocontrol Pseudomonas chlororaphis O6 Zinc ZnO nanoparticles
This work was supported by the United States Department of Agriculture (USDA-CSREES Grant 2011-03581), the Utah Water Research Laboratory, and the Utah Agricultural Experimental Station (Journal Paper Number 8551).
- Aydin SB, Hanley L (2010) Antibacterial activity of dental composites containing zinc oxide nanoparticles. J Biomed Mater Res B 94:22–31Google Scholar
- Cook RJ, Veseth RJ (1991) Wheat health management. Plant Heath Management Series. American Phytopathology Society Press, St. PaulGoogle Scholar
- Dimkpa CO, Zeng J, McLean JE, Britt DW, Zhan J, Anderson AJ (2012b) Production of indole-3-acetic acid via the indole-3-acetamide pathway in the plant-beneficial bacterium Pseudomonas chlororaphis O6 is inhibited by ZnO nanoparticles but enhanced by CuO nanoparticles. Appl Environ Microbiol 78:1404–1410PubMedCrossRefGoogle Scholar
- Emami-Karvani Z, Chehrazi P (2011) Antibacterial activity of ZnO nanoparticle on gram-positive and gram-negative bacteria. Afr J Microbiol Res 5:1368–1373Google Scholar
- Gilchrist L, Dubin HJ (2002) Fusarium head blight. In: Curtis BC, Rajaram S, Gómez Macpherson H (eds) Bread wheat improvement and production. FAO Plant Production and Protection Series No. 30. Food and Agriculture Organization of the United Nations, RomeGoogle Scholar
- Jayaseelan C, Abdul Rahuman A, Kirthi AV, Marimuthu S, Santhoshkumar T, Bagavan A, Gaurav K, Karthik L, Bhaskara-Rao KV (2012) Novel microbial route to synthesize ZnO nanoparticles using Aeromonas hydrophila and their activity against pathogenic bacteria and fungi. Spectrochim Acta A Mol Biomol Spectrosc 90:78–84PubMedCrossRefGoogle Scholar
- Kang BR, Han SH, Zdor RE, Anderson AJ, Spencer M, Yang KY, Kim YH, Lee MC, Cho BH, Kim YC (2007) Inhibition of seed germination and induction of systemic disease resistance by Pseudomonas chlororaphis O6 requires phenazine production regulated by the global regulator, gacS. J Microbiol Biotechnol 17:586–593PubMedGoogle Scholar
- López-Moreno ML, de la Rosa G, Hernández-Viezcas JA, Castillo-Michel H, Botez CE, Peralta-Videa JR, Gardea-Torresdey JL (2010) Evidence of the differential biotransformation and genotoxicity of ZnO and CeO2 nanoparticles on soybean (Glycine max) plants. Environ Sci Technol 44:7315–7320PubMedCrossRefGoogle Scholar
- Priester JH, Ge Y, Mielke RE, Horst AM, Moritz SC, Espinosa K, Gelb J, Walker SL, Nisbet RM, An Y-J, Schimel JP, Palmer RG, Hernandez-Viezcas JA, Zhao L, Gardea-Torresdey JL, Holden PA (2012) Soybean susceptibility to manufactured nanomaterials with evidence for food quality and soil fertility interruption. Proc Natl Acad Sci USA 109:2451–2456CrossRefGoogle Scholar
- Stampoulis D, Sinha SK, White JC (2009) Assay-dependent phytotoxicity of nanoparticles to plants. Environ Sci Technol 43:9473–9479Google Scholar
- Xie Y, He Y, Irwin PL, Jin T, Shi X (2011) Antibacterial activity and mechanism of action of zinc oxide nanoparticles against Campylobacter jejuni. Appl Environ Microbiol 77:325–2331Google Scholar