, Volume 26, Issue 1, pp 189–196 | Cite as

The effect of temperature on antibacterial activity of biosynthesized silver nanoparticles

  • Parastoo Pourali
  • Majid Baserisalehi
  • Sima Afsharnezhad
  • Javad Behravan
  • Rashin Ganjali
  • Nima Bahador
  • Sepideh Arabzadeh


The purpose of this study was the evaluation of two different temperatures on antibacterial activity of the biosynthesized silver nanoparticles. 38 silver nanoparticles-producing bacteria were isolated from soil and identified. Biosynthesis of silver nanoparticles by these bacteria was verified through visible light spectrophotometry. Two strains were relatively active for production of silver nanoparticles. These strains were subjected for molecular identification and recognized as Bacillus sp. and Acinetobacter schindleri. In the present study, the effect of temperatures was evaluated on structure and antimicrobial properties of the silver nanoparrticles by transmission electron microscopy (TEM), X-ray diffraction (XRD) analysis and antimicrobial Agar well diffusion methods. The silver nanoparticles showed antibacterial activity against all the pathogenic bacteria; however, this property was lost after treatment of the silver nanoparticles by high temperatures (100 and 300 °C). TEM images showed that the average sizes of heated silver nanoparticles were >100 nm. However, these were <100 nm for non-heated silver nanoparticles. Although, XRD patterns showed the crystalline structure of heated silver nanoparticles, their antibacterial activities were less. This was possible because of the sizes and accordingly less penetration of the particles into the bacterial cells. In addition, elimination of the capping agents by heat might be considered another reason.


Biosynthesized Silver nanoparticles Temperature Antibacterial activity 


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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Parastoo Pourali
    • 1
  • Majid Baserisalehi
    • 2
  • Sima Afsharnezhad
    • 3
  • Javad Behravan
    • 4
  • Rashin Ganjali
    • 5
  • Nima Bahador
    • 1
  • Sepideh Arabzadeh
    • 4
  1. 1.Department of Microbiology, Science and Research BranchIslamic Azad UniversityFarsIran
  2. 2.Department of Microbiology, Kazeroun BranchIslamic Azad UniversityKazerounIran
  3. 3.Department of Biochemistry, Mashhad BranchIslamic Azad UniversityMashhadIran
  4. 4.Biotechnology Research CenterMashhad University of Medical SciencesMashhadIran
  5. 5.Immunology Research CenterMashhad University of Medical SciencesMashhadIran

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