BioMetals

, Volume 24, Issue 6, pp 1059–1067 | Cite as

Pseudomonas siderophores in the sputum of patients with cystic fibrosis

  • Lois W. Martin
  • David W. Reid
  • Katrina J. Sharples
  • Iain L. Lamont
Article

Abstract

The lungs of patients with cystic fibrosis become chronically infected with the bacterium Pseudomonas aeruginosa, which heralds progressive lung damage and a decline in health. Iron is a crucial micronutrient for bacteria and its acquisition is a key factor in infection. P. aeruginosa can acquire this element by secreting pyoverdine and pyochelin, iron-chelating compounds (siderophores) that scavenge iron and deliver it to the bacteria. Siderophore-mediated iron uptake is generally considered a key factor in the ability of P. aeruginosa to cause infection. We have investigated the amounts of pyoverdine in 148 sputum samples from 36 cystic fibrosis patients (30 infected with P. aeruginosa and 6 as negative controls). Pyoverdine was present in 93 samples in concentrations between 0.30 and 51 μM (median 4.6 μM) and there was a strong association between the amount of pyoverdine and the number of P. aeruginosa present. However, pyoverdine was not present, or below the limits of detection (~0.3 μM), in 21 sputum samples that contained P. aeruginosa. Pyochelin was also absent, or below the limits of detection (~1 μM), in samples from P. aeruginosa-infected patients with little or no detectable pyoverdine. Our data show that pyoverdine is an important iron-scavenging molecule for P. aeruginosa in many cystic fibrosis patients, but other P. aeruginosa iron-uptake systems must be active in some patients to satisfy the bacterial need for iron.

Keywords

Pyoverdine Pyochelin Iron and infection Pseudomonas aeruginosa Cystic fibrosis 

Abbreviation

CF

Cystic fibrosis

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Copyright information

© Springer Science+Business Media, LLC. 2011

Authors and Affiliations

  • Lois W. Martin
    • 1
  • David W. Reid
    • 3
    • 4
  • Katrina J. Sharples
    • 2
  • Iain L. Lamont
    • 1
  1. 1.Department of BiochemistryUniversity of OtagoDunedinNew Zealand
  2. 2.Department of Preventive and Social MedicineUniversity of OtagoDunedinNew Zealand
  3. 3.Menzies Research Institute and University of Tasmania Medical SchoolHobartAustralia
  4. 4.Department of Thoracic MedicineThe Prince Charles HospitalChermside, BrisbaneAustralia

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