BioMetals

, Volume 24, Issue 2, pp 323–333 | Cite as

Characterization and expression of chicken selenoprotein W

  • Bor-Rung Ou
  • Mei-Jung Jiang
  • Chao-Hsiang Lin
  • Yu-Chuan Liang
  • Kuei-Jen Lee
  • Jan-Ying Yeh
Article
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Abstract

As an essential trace element, selenium (Se) deficiency results in White Muscle Disease in livestock and Keshan disease in humans. The main objectives of this study were to clone and characterize the chicken selenoprotein W (SeW) gene and investigate SeW mRNA expression in chicken tissues. The deduced amino acid (AA) sequence of chicken SeW contains 85 AAs with UAG as the stop codon. Like all SeW genes identified in different species, chicken SeW contains one well-conserved selenocysteine (Sec) at the 13th position encoded by the UGA codon. The proposed glutathione (GSH)-binding site at the Cys37 of SeW is not conserved in the chicken, but Cys9 and Sec13, with possible GSH binding, are conserved in SeWs identified from all species. There are 23–59% and 50–61% homology in cDNA and deduced AA sequences of SeW, respectively, between the chicken and other species. The predicted secondary structure of chicken SeW mRNA indicates that the selenocysteine insertion sequence element is type II with invariant adenosines within the apical bulge. The SeW mRNA expression is high in skeletal muscle followed by brain, but extremely low in other tissues from chickens fed a commercial maize-based diet. The SeW gene is ubiquitously expressed in heart, skeletal muscle, brain, testis, spleen, kidney, lung, liver, stomach and pancreas in chickens fed a commercial diet supplemented with sodium selenite. These results indicate that dietary selenium supplementation regulates SeW gene expression in the chicken and skeletal muscle is the most responsive tissue when dietary Se content is low.

Keywords

Selenoprotein W mRNA expression Selenium cDNA sequence Chicken 
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Notes

Acknowledgements

We thank Ms. Judy A. Butler from Linus Pauling Institute in Corvallis, Oregon USA for her critical reading of this manuscript. This research was supported by National Science Council in Taiwan, ROC (NSC93-2313-B-468-005 and NSC95-2313-B-468-002-MY2).

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Copyright information

© Springer Science+Business Media, LLC. 2011

Authors and Affiliations

  • Bor-Rung Ou
    • 1
  • Mei-Jung Jiang
    • 2
  • Chao-Hsiang Lin
    • 2
  • Yu-Chuan Liang
    • 2
    • 3
  • Kuei-Jen Lee
    • 4
  • Jan-Ying Yeh
    • 2
  1. 1.Department of Animal Science and BiotechnologyTunghai UniversityTaichungTaiwan, ROC
  2. 2.Department of BiotechnologyAsia UniversityTaichungTaiwan, ROC
  3. 3.Agricultural Biotechnology Research Center, Academia SinicaTaipeiTaiwan, ROC
  4. 4.Department of BioinformaticsAsia UniversityTaichungTaiwan, ROC

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