BioMetals

, Volume 24, Issue 3, pp 513–522

Iron acquisition with the natural siderophore enantiomers pyochelin and enantio-pyochelin in Pseudomonas species

  • Zeb A. Youard
  • Nicolas Wenner
  • Cornelia Reimmann
Article

DOI: 10.1007/s10534-010-9399-9

Cite this article as:
Youard, Z.A., Wenner, N. & Reimmann, C. Biometals (2011) 24: 513. doi:10.1007/s10534-010-9399-9

Abstract

The bacterial siderophore pyochelin is composed of salicylate and two cysteine-derived heterocycles, the second of which is modified by reduction and N-methylation during biosynthesis. In Pseudomonas aeruginosa, the first cysteine residue is converted to its D-isoform during thiazoline ring formation, whereas the second cysteine remains in its L-configuration. Stereochemistry is opposite in the Pseudomonas fluorescens siderophore enantio-pyochelin, in which the first ring originates from L-cysteine and the second ring from D-cysteine. Both siderophores promote growth of the producer organism during iron limitation and induce the expression of their biosynthesis genes by activating the transcriptional AraC-type regulator PchR. However, neither siderophore is functional as an iron carrier or as a transcriptional inducer in the other species, demonstrating that both processes are highly stereospecific. Stereospecificity of pyochelin/enantio-pyochelin-mediated iron uptake is ensured at two levels: (i) by the outer membrane siderophore receptors and (ii) by the cytosolic PchR regulators.

Keywords

Siderophore Pseudomonas Enantiomer Pyochelin Enantio-pyochelin Stereospecificity 

Copyright information

© Springer Science+Business Media, LLC. 2010

Authors and Affiliations

  • Zeb A. Youard
    • 1
  • Nicolas Wenner
    • 1
  • Cornelia Reimmann
    • 1
  1. 1.Département de Microbiologie FondamentaleUniversité de LausanneLausanneSwitzerland

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