, Volume 24, Issue 1, pp 105–115 | Cite as

Protective effect of ascorbic acid on cadmium-induced hypertension and vascular dysfunction in mice

  • Wanida Donpunha
  • Upa KukongviriyapanEmail author
  • Kwanjit Sompamit
  • Poungrat Pakdeechote
  • Veerapol Kukongviriyapan
  • Patchareewan Pannangpetch


Cadmium (Cd) is one of the most important environmental pollutants that cause a number of adverse health effects in humans and animals. Recent studies have shown that Cd-induced oxidative damage within the vascular tissues results in vascular dysfunction. The current study was aimed to investigate whether ascorbic acid could protect against Cd-induced vascular dysfunction in mice. Male ICR mice were received CdCl2 (100 mg/l) via drinking water for 8 weeks alone or received ascorbic acid supplementation at doses of 50 and 100 mg/kg/day for every other day. Results showed that Cd administration increased arterial blood pressure and blunted the vascular responses to vasoactive agents. These alterations were related to increased superoxide production in thoracic aorta, increased urinary nitrate/nitrite, increased plasma protein carbonyl, elevated malondialdehyde (MDA) concentrations in plasma and tissues, decreased blood glutathione (GSH), and increased Cd contents in blood and tissues. Ascorbic acid dose-dependently normalized the blood pressure, improved vascular reactivities to acetylcholine (ACh), phenylephrine (Phe) and sodium nitroprusside (SNP). These improvements were associated with significant suppression of oxidant formation, prevention of GSH depletion, and partial reduction of Cd contents in blood and tissues. The findings in this study provide the first evidence in pharmacological effects of ascorbic acid on alleviation of oxidative damage and improvement of vascular function in a mouse model of Cd-induced hypertension and vascular dysfunction. Moreover, our study suggests that dietary supplementation of ascorbic acid may provide beneficial effects by reversing the oxidative stress and vascular dysfunction in Cd-induced toxicity.


Ascorbic acid Cadmium Hypertension Oxidative stress Vascular dysfunction 



This work was supported by the Faculty of Medicine, and the Graduate School Research Funds, Khon Kaen University, Thailand. Wanida Donpunha was supported by a Ph.D. scholarship from Graduate School, Khon Kaen University, Thailand.


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Copyright information

© Springer Science+Business Media, LLC. 2010

Authors and Affiliations

  • Wanida Donpunha
    • 1
    • 2
  • Upa Kukongviriyapan
    • 1
    Email author
  • Kwanjit Sompamit
    • 3
  • Poungrat Pakdeechote
    • 1
  • Veerapol Kukongviriyapan
    • 4
  • Patchareewan Pannangpetch
    • 4
  1. 1.Department of Physiology, Faculty of MedicineKhon Kaen UniversityKhon KaenThailand
  2. 2.Medical Physiology Graduate Program, Graduate SchoolKhon Kaen UniversityKhon KaenThailand
  3. 3.Faculty of MedicineMahasarakham UniversityMahasarakhamThailand
  4. 4.Department of Pharmacology, Faculty of MedicineKhon Kaen UniversityKhon KaenThailand

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