Effects of tellurite on growth of Saccharomyces cerevisiae
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The effects of potassium tellurite on growth and survival of rho+ and rho0 Saccharomyces cerevisiae strains were investigated. Both rho+ and rho0 strains grew on a fermentable carbon source with up to 1.2 mM K2TeO3, while rho+ yeast cells grown on a non-fermentable carbon source were inhibited at tellurite levels as low as 50 μM suggesting that this metalloid specifically inhibited mitochondrial functions. Growth of rho+ yeast cells in the presence of increasing amount of tellurite resulted in dose-dependent blackening of the culture, a phenomenon not observed with rho0 cultures. Transmission electron microscopy of S. cerevisiae rho+ cells grown in the presence of tellurite showed that blackening was likely due to elemental tellurium (Te0) that formed large deposits along the cell wall and small precipitates in both the cytoplasm and mitochondria.
KeywordsTellurite Saccharomyces cerevisiae Mitochondria Transmission electronic microscopy
The research was supported by Compagnia di San Paolo special grant “iniziativa” to L. Del Giudice. P. Pontieri was supported by a postdoctoral grant from the Istituto Banco di Napoli, Fondazione.
- Baesman SM, Bullen TD, Dewald J, Zhang D, Curran S, Islam FS, Beveridge TJ, Oremland RS (2007) Formation of tellurium nanocrystals during anaerobic growth of bacteria that use Te oxyanion as respiratory electron acceptors. Appl Environ Microbiol 73:2135–2143. doi: 10.1128/AEM.02558-06 CrossRefPubMedGoogle Scholar
- Borghese R, Borsetti F, Foladori P, Ziglio G, Zannoni D (2004) Effects of the metalloid oxyanion tellurite (TeO3 2−) on growth characteristics of the phototrophic bacterium Rhodobacter capsulatus. Appl Environ Microbiol 70:6595–6602. doi: 10.1128/AEM.70.11.6595-6602.2004 CrossRefPubMedGoogle Scholar
- Cooper WC (1971) Tellurium. Van Nostrand Renhod Co, New YorkGoogle Scholar
- Del Giudice L, Massardo DR, Pontieri P, Wolf K (2005) Interaction between yeast mitochondrial and nuclear genomes: Null alleles of RTG genes affect resistance to the alkaloid lycorine in rho0 petites of Saccharomyces cerevisiae. Gene 354:9–14. doi: 10.1016/j.gene.2005.03.020 CrossRefPubMedGoogle Scholar
- Gharieb MM, Gadd GM (1998) Evidence for the involvement of vacuolar activity in metal (loid) tolerance: vacuolar-lacking and -defective mutants of Saccharomyces cerevisiae display higher sensitivity to chromate, tellurite and selenite. Biometals 11:101–106. doi: 10.1023/A:1009221810760 CrossRefPubMedGoogle Scholar