The effects of lactoferrin in a rat model of catecholamine cardiotoxicity
- 147 Downloads
Lactoferrin is recently under intense investigation because of its proposed several pharmacologically positive effects. Based on its iron-binding properties and its physiological presence in the human body, it may have a significant impact on pathological conditions associated with iron-catalysed reactive oxygen species (ROS). Its effect on a catecholamine model of myocardial injury, which shares several pathophysiological features with acute myocardial infarction (AMI) in humans, was examined. Male Wistar rats were randomly divided into four groups according to the received medication: control (saline), isoprenaline (ISO, 100 mg kg−1 s.c.), bovine lactoferrin (La, 50 mg kg−1 i.v.) or a combination of La + ISO in the above-mentioned doses. After 24 h, haemodynamic functional parameters were measured, a sample of blood was withdrawn and the heart was removed for analysis of various parameters. Lactoferrin premedication reduced some impairment caused by ISO (e.g. a stroke volume decrease, an increase in peripheral resistance and calcium overload). These positive effects were likely to have been mediated by the positive inotropic effect of lactoferrin and by inhibition of ROS formation due to chelation of free iron. The failure of lactoferrin to provide higher protection seems to be associated with the complexity of catecholamine cardiotoxicity and with its hydrophilic character.
KeywordsLactoferrin Isoprenaline Iron chelators Reactive-oxygen species Iron Catecholamines
The authors wish to thank to Mrs. Anezka Kunova for her excellent technical support. This work was supported by grants of Charles University, No. 94/2006/C/FaF and 39207 C and by Research Project, No. MZO 001179906.
- Bennett RM, Kokocinski T (1979) Lactoferrin turnover in man. Clin Sci (Lond) 57:453–460Google Scholar
- Chagoya de Sanchez V, Hernandez-Munoz R, Lopez-Barrera F, Yanez L, Vidrio S, Suarez J, Cota-Garza MD, Aranda-Fraustro A, Cruz D (1997) Sequential changes of energy metabolism and mitochondrial function in myocardial infarction induced by isoproterenol in rats: a long-term and integrative study. Can J Physiol Pharmacol 75:1300–1311. doi: 10.1139/cjpp-75-12-1300 PubMedCrossRefGoogle Scholar
- Lentner C (1990) Geigy scientific tables. Ciba-Geigy, BaselGoogle Scholar
- Neri M, Cerretani D, Fiaschi AI, Laghi PF, Lazzerini PE, Maffione AB, Micheli L, Bruni G, Nencini C, Giorgi G, D’Errico S, Fiore C, Pomara C, Riezzo I, Turillazzi E, Fineschi V (2007) Correlation between cardiac oxidative stress and myocardial pathology due to acute and chronic norepinephrine administration in rats. J Cell Mol Med 11:156–170. doi: 10.1111/j.1582-4934.2007.00009.x PubMedCrossRefGoogle Scholar
- Parkkinen J, Sahlstedt L, von Bonsdorff L, Salo H, Ebeling F, Ruutu T (2006) Effect of repeated apotransferrin administrations on serum iron parameters in patients undergoing myeloablative conditioning and allogeneic stem cell transplantation. Br J Haematol 135:228–234. doi: 10.1111/j.1365-2141.2006.06273.x PubMedCrossRefGoogle Scholar
- Plomteux G, Charlier C, Albert A, Farnier M, Pressac M, Vernet M, Paris M, Dellamonica C, Dezier JF (1987) Reference values of serum transferrin in newborn infants, children and adults. Ann Biol Clin (Paris) 45:622–629Google Scholar
- Sterba M, Popelova O, Simunek T, Mazurova Y, Potacova A, Adamcova M, Guncova I, Kaiserova H, Palicka V, Ponka P, Gersl V (2007) Iron chelation-afforded cardioprotection against chronic anthracycline cardiotoxicity: a study of salicylaldehyde isonicotinoyl hydrazone (SIH). Toxicology 235:150–166. doi: 10.1016/j.tox.2007.03.020 PubMedCrossRefGoogle Scholar