BioMetals

, Volume 21, Issue 6, pp 613–621 | Cite as

Responses of Rhodotorula sp. Y11 to cadmium

Article

Abstract

Some aspects of the cellular responses to cadmium were extensively investigated in the yeast Rhodotorula sp. Y11. Scanning electron microscopy indicated that accumulation of cadmium in the Y11 did not cause any visible effects on cell morphology. More than 20% yeast cells still showed viability after 15 h of cadmium accumulation under 100 mg l−1 cadmium concentration, and transmission electron microscopy analysis showed that plasmolysis and thickened cell wall were not observed in all of the cells. In the presence of cadmium, the activities of superoxide dismutase (SOD) and catalase (CAT) were all greater than the control, but the increase was in a dose-independent manner. Changes in SOD and CAT activities were also dependent on the time of exposure. Therefore, it suggests that antioxidative defenses play an important role in cadmium tolerance in Rhodotorula sp. Y11. Nondenaturing polyacrylamide gels revealed only one SOD isoforms in Y11 even under exposure to cadmium.

Keywords

Cadmium Catalase Rhodotorula sp. Resistance Superoxide dismutase 

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Copyright information

© Springer Science+Business Media, LLC. 2008

Authors and Affiliations

  1. 1.College of Biological Sciences, Key Laboratory of Agro-Microbial Resource and Application, Ministry of AgricultureChina Agricultural UniversityBeijingPeople’s Republic of China

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