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BioMetals

, Volume 20, Issue 1, pp 27–36 | Cite as

Effect of nickel on ROS content and antioxidative enzyme activities in wheat leaves

  • Ewa GajewskaEmail author
  • Maria Skłodowska
Article

Abstract

Influence of 100 μM Ni on growth, Ni accumulation,\(\ \hbox{O}_{2}^{\cdot-}\), H2O2 and lipid peroxides contents as well as the activities of superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), guaiacol peroxidase (POD) and glutathione peroxidase (GSH-Px) were studied in the leaves of wheat plants on the 3rd, 6th and 9th days after treatment. Exposure of the plants to Ni for only 3 days led to almost 200-fold increase in this metal concentration in the leaf tissue but later the rate of Ni accumulation was much slower. Length and fresh weight of the leaves were substantially reduced, up to 25% and 39%, respectively at the end of experiment. Visible symptoms of Ni toxicity: chlorosis and necrosis were observed following the 3rd day. Treatment with Ni resulted in the increase in\( \ \hbox{O}_{2}^{\cdot -}\) and H2O2 contents in the leaves. Both showed their highest values, approximately 250% of those of the control, on the 3rd day and then their levels decreased but still markedly exceeded the control values. SOD and CAT activities decreased significantly in response to Ni treatment, however a several-fold increase in APX and POD activities was found. No significant changes in lipid peroxides content were observed in the leaves after Ni application. The activity of GSH-Px showed a 29% induction on the 3rd day. Our results indicated that despite prolonged increases in\( \ \hbox{O}_{2}^{\cdot-}\) and H2O2 levels, oxidative damage, measured as the level of lipid peroxidation, did not occur in the leaves of Ni-treated wheat.

Keywords

antioxidative enzymes lipid peroxidation nickel reactive oxygen species Triticum aestivum L. 

Abbreviations

APX

ascorbate peroxidase

CAT

catalase

GSH

reduced glutathione

GSH-Px

glutathione peroxidase

MBTH

3-methyl-2-benzothiazolinone hydrazone

NBT

nitro blue tetrazolium

POD

guaiacol peroxidase

ROS

reactive oxygen species

SOD

superoxide dismutase

TBARS

thiobarbituric acid reacting substances

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Notes

Acknowledgements

This work was partly supported by University of Łódź Grant No. 505/429. The authors are grateful to Dr. Z. Nita (Hodowla Roślin Strzelce Sp. z o.o., Poland) for supplying the wheat seeds.

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Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  1. 1.Department of Plant Physiology and BiochemistryUniversity of ŁódźŁódźPoland

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