, Volume 19, Issue 1, pp 71–81 | Cite as

Magnesium Deficiency Enhances Hydrogen Peroxide Production and Oxidative Damage in Chick Embryo Hepatocyte In Vitro

  • Ying Yang
  • Zhenlong Wu
  • Yue Chen
  • Jian Qiao
  • Mingyu Gao
  • Jianmin Yuan
  • Wei Nie
  • Yuming Guo


Magnesium deficiency and oxidative stress have been identified as correlative factors in many diseases. The origin of free radicals correlated with oxidative damage resulting from Mg-deficiency is unclear at the cellular level. To investigate whether hydrogen peroxide (H2O2) is associated in the oxidative stress induced by Mg-deficiency, the effect of Mg2+ deficiency (0, 0.4, 0.7 mM) on the metabolism of H2O2 was investigated in cultured chick embryo hepatocytes. After being cultured in the media with various concentrations of Mg2+ for 1, 2, 4, 6 and 10 days, parameters of H2O2 production, catalase activity, lipid peroxidation, intracellular total Mg and cell viability were analyzed. Results demonstrated that long-term incubation of chick embryo hepatocyte in extracellular Mg2+-deprivative and Mg2+-deficient (0.4 mM) states significantly enhanced the production of H2O2 (approximately twofold, respectively) and lipid peroxidation in the cell cultures, while decreasing the cell viability. Additionally, the reversing action of Mg2+ re-added to 1.0 mM and the partial reversing action of dimethylthiourea suggested that (i) [Mg2+]e deficiency induced the increase of H2O2 production, (ii) [Mg2+]e deficiency decreased catalase activity in chick embryo hepatocyte in vitro, subsequently causing oxidative stress and cell peroxidative damage.


chick embryo catalase hepatocyte hydrogen peroxide Magnesium 


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Copyright information

© Springer 2006

Authors and Affiliations

  • Ying Yang
    • 1
  • Zhenlong Wu
    • 2
  • Yue Chen
    • 3
  • Jian Qiao
    • 4
  • Mingyu Gao
    • 4
  • Jianmin Yuan
    • 1
  • Wei Nie
    • 1
  • Yuming Guo
    • 1
  1. 1.Division of Animal Nutrition and Feed Science, College of Animal Science and TechnologyChina Agricultural University (CAU)BeijingP.R. China
  2. 2.Division of Animal Physiology, College of Biological SciencesCAUBeijingP.R. China
  3. 3.National Natural Science Foundation of ChinaBeijingP.R. China
  4. 4.Division of Basic Veterinary Medicine, College of Veterinary MedicineCAUBeijingP.R. China

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